UltraScence Femto Plus Western Substrate,作為一種基于發(fā)光氨的增強(qiáng)化學(xué)發(fā)光底物,是敏感的,與辣根過氧化物酶(HRP)偶聯(lián)二抗體進(jìn)行免疫印跡兼容。UltraScence Femto Plus Western底物的設(shè)計(jì)目的是檢測數(shù)量太小的目標(biāo)蛋白,用典型的ECL底物看不到。UltraScence Femto Plus Western Substrate的卓越靈敏度和長信號持續(xù)時間使抗原的低飛圖檢測(中飛波)成為可能。此外,它的長化學(xué)發(fā)光信號持續(xù)時間使數(shù)字和基于薄膜的成像不損失任何信號成為可能。建議適當(dāng)?shù)囊豢购投瓜♂屢垣@得最佳的信號強(qiáng)度和持續(xù)時間。此外,UltraScence Femto Plus WesternSubstrate的高穩(wěn)定性使其在室溫下可以存儲2年,而不會對性能產(chǎn)生任何影響。
產(chǎn)品描述
The UltraScence Femto Plus Western Substrate, as a luminol-based enhanced chemiluminescent substrate,is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugatedsecondary antibodies. The UltraScence Femto Plus Western Substrate is designed for the detection of the targetproteins in amounts that are too small to be seen with typical ECL substrates. The low femtogram detection (midzeptomole) of antigen is enabled by UltraScence Femto Plus Western Substrate’s excellent sensitivity and longsignal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imagingpossible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested forattaining optimal signal intensity and duration. Moreover, the high stability of UltraScence Femto Plus WesternSubstrate makes a 2-year- storage at room temperature possible, without any compromise on the performance.