大內(nèi)皮素Elisa試劑盒-Big Endothelin-1 ELISA Kit代理/參數(shù)/廠家-ELISA試劑盒-艾美捷科技有限公司

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背景資料: Big Endothelin-1 (BigET-1) is a peptide of 38 amino acids and is the precursor of Endothelin-1 (ET-1), consisting of amino acids 1-21 (http://www.uniprot.org/uniprot/P05305). ET-1 is a potent vasoconstrictor and is produced by vascular endothelial cells. Accordingly, it has a wide tissue distribution (http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.511899). The cleavage of BigET-1 by the Endothelin-converting enzyme (ECE) converts BigET-1 to Endothelin (ET) and to a C?terminal fragment.

The half-life of ET-1 (1-21) in plasma is less than one minute, which may preclude its use as a diagnostic marker, whereas clearance of the precursor peptide BigET-1 is much slower. As Big-Endothelin-1 is produced at amounts equimolar to ET-1 and has higher plasma stability, it is more suitable for diagnostics and monitoring of heart failure (Gergei et al., 2018). Hence, Big ET-1 has the same physiological role as ET-1 while being a more reliable marker.

產(chǎn)品描述: Method Sandwich ELISA, HRP/TMB, 12×8-well detachable strips Sample type Serum, EDTA plasma. citrate plasma, hepar?n plasma Sample volume 50 μl / well Assay time 4 h / 1 h / 30 min Sensitivity 0.02 pmol/l (= 0.086 pg/ml) Standard range 0 – 3 pmol/l (= 0 – 12.85 pg/ml) Conversion factor 1 pmol/l = 4.283 pg/ml (MW: 4.283 kDa) Precision In-between-run (n=5): ≤ 5 % CV Within-run (n=10): ≤ 4 % CV Specificity Endogenous and recombinant Big Endothelin-1 (Big ET-1 (1-38)). Cross-reactivity Human ET1/2/3 (1-21): <1%, human ET2 (1-37): <1%, human ET1/2 (1-38): <1%, human BigET1/2 (22-38) : <1%, human BigET2 (22-37) : <1%, porcine BigET (1-39): 21%, rat BigET1 (1-39): 10%, Sarafotoxin: <1%

其他: Biomedica Immunoassay(bmgrp)公司成立于1988年, 總部位于奧地利維也納，主要致力于研究、開(kāi)發(fā)和生產(chǎn)基于ELISA方法用于臨床前研究相關(guān)標(biāo)記物檢測(cè)的工具。迄今為止Biomedica Immunoassay公司在心血管檢測(cè)及骨代謝檢測(cè)領(lǐng)域開(kāi)發(fā)了一系列特色ELISA檢測(cè)試劑盒，Biomedica Immunoassay的生產(chǎn)過(guò)程符合GMP/GLP指南，并符合ISO 9001:2015管理體系，有CE認(rèn)證。Biomedica Immunoassay的產(chǎn)品根據(jù)FDA， EMEA和ICH指南進(jìn)行驗(yàn)證，并具有驗(yàn)證報(bào)告。