C–type natriuretic peptide (CNP) is a paracrine growth factor widely expressed in various tissues. The 103-amino acid propeptide is cleaved either between residues 50 and 51 or 81 and 82 to produce one of two biologically active peptides, carboxy-terminal proCNP (51–103) or carboxy-terminal proCNP (82–103), respectively, and an amino-terminal congener, N-terminal proCNP.
產(chǎn)品描述
Method
Sandwich ELISA, HRP/TMB, 12×8-well detachable strips
Sample type
Serum, EDTA plasma, citrate plasma, hepar?n plasma, urine, cell culture supernatant, human and non-human samples
Assay time
20 min / 3 h / 30 min
Sample volume
20 μl / well
Sensitivity
0.7 pmol/l (= 3.49 pg/ml)
Standard range
0 – 128 pmol/l (= 0 – 638 pg/ml)
Conversion factor
1 pmol/l = 4.985 pg/ml (MW: 4.985 kDa)
Specificity
This assay recognizes recombinant and endogenous NT-proCNP.
Precision
In-between-run (n=8): ≤ 7 % CV
Within-run (n=5): ≤ 6 % CV