人白介素6 Elisa試劑盒-Human IL-6 ELISA Kit代理/參數(shù)/廠家-ELISA試劑盒-艾美捷科技有限公司

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中文名稱: 人白介素6 Elisa試劑盒

英文名字: Human IL-6 ELISA Kit

供應商: Biomedica Immunoassays

產(chǎn)品貨號: BI-IL6

產(chǎn)品報價: ￥12x8tests

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背景資料: Interleukin-6 (IL-6), also known as B-cell stimulatory factor 2 (BSF-2), CTL differentiation factor (CDF), Hybridoma growth factor or Interferon beta-2 (IFN-beta-2), was successfully cloned by Hirano et al. in 1986 (Hirano T et al.) . The gene is mapped at chromosome 7p21. IL-6 protein is built up by 183 amino acids and has a calculated molecular weight of 23.7 kDa.

It is a pleiotropic, alpha helical protein that is composed of a four-helix bundle (Somers W et al.). It shares 39% sequence identity with mouse and 40% with rat IL-6. IL-6 is phosphorylated at amino acid 81 and it is variably glycosylated by N-linked glycosylation. IL-6 belongs to the IL-6/GCSF/MGF protein family (Rose-John S et al.) whose members share a common use of the gp130 receptor subunit. IL-6 isoforms, with internal deletions, are generated by alternative splicing. The principal cell sources for IL-6 are mononuclear phagocytes, vascular endothelial cells, fibroblasts or other cells.

IL-6 is the ligand for the Interleukin-6 receptor α (IL-6Rα) (Schwantner A et al.) that occurs membrane-bound, but that may also circulate as soluble form generated by alternative splicing or proteolytic cleavage. To induce signaling, IL-6 first forms a complex with the non-signaling IL-6Rα. Subsequent binding to the signal transducing subunit gp130 leads to dimerization of gp130 and finally to the formation of the hexameric signaling complex (Boulanger MJ et al.).

Complexes of IL-6 and soluble IL-6Rα may elicit responses in cells lacking the membrane-bound IL-6Rα but expressing the ubiquitous gp130 coreceptor. This process is known as trans-signaling, it enlarges the spectrum of target cells responding to IL-6 (Mihara M et al.).

產(chǎn)品描述: Method Sandwich ELISA, HRP/TMB, 12×8-well detachable strips Sample type Serum, plasma (EDTA, citrate, heparin), cell culture supernatants, urine Sample volume 100 μl sample / well Assay time 2 h / 1 h / 1h /30 min Sensitivity LOD: 0.28 pg/ml; LLOQ: 0.78 pg/ml (measurable concentrations in serum AND plasma samples!) Standard range 0 – 200 pg/ml (0 / 3.12 / 6.25 / 12.5 / 25/ 50 / 100 / 200) Precision In-between-run (n=3): ≤ 6 % CV Within-run (n=3): ≤ 7 % CV Specificity This assay recognizes recombinant and endogenous (natural) human IL-6 Validation Data See validation data tab for: precision, accuracy, dilution linearity, values for healthy donors, etc

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保存建議: +4°C

其他: Biomedica Immunoassay(bmgrp)公司成立于1988年, 總部位于奧地利維也納，主要致力于研究、開發(fā)和生產(chǎn)基于ELISA方法用于臨床前研究相關標記物檢測的工具。迄今為止Biomedica Immunoassay公司在心血管檢測及骨代謝檢測領域開發(fā)了一系列特色ELISA檢測試劑盒，Biomedica Immunoassay的生產(chǎn)過程符合GMP/GLP指南，并符合ISO 9001:2015管理體系，有CE認證。Biomedica Immunoassay的產(chǎn)品根據(jù)FDA， EMEA和ICH指南進行驗證，并具有驗證報告。