中文名稱

激酶 RR 試劑盒

英文名字

Kinase RR Kit

供應(yīng)商

BioThema

產(chǎn)品貨號

BTA-111-021

產(chǎn)品報(bào)價(jià)

￥詢價(jià)/1000tests

產(chǎn)品說明書

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購買方式

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產(chǎn)品新聞

背景資料

Kinase RR Kit is based on a method of measuring the first order reaction rate constant of protein kinase reactions by monitoring the ATP degradation rate using bioluminescence. The assay starts by adding ATP to a reaction mixture containing all necessary assay components, including luciferase and luciferin. In HTS the light is measured at two points in time. In non-HTS the light can be measured repeatedly until a significan reduction of light is obtained.nnThe assay is set up so that:na) the kinase reaction follows first order kineticsnnb) the sample light emission, normalized against the blank (no kinase), is proportional to the remaining ATP concentration.nnThe first order rate constant of the kinase reaction is proportional to the kinase activity over several orders of magnitude, which makes it very easy to set up new assays. Furthermore variation in ATP and luciferase cancel out in calculations and do not affect assay results.

產(chǎn)品描述

Kinase RR Kit or Kinase Reaction rate Kit is developed for the easy and reliable identification of protein kinase inhibitor candidates from large compund libraries. The Kinase RR Kit uses direct bioluminescent measurement of ATP degradation rate to distingusih hits from non-hits. In contrast to other bioluminescent ATP based assays of kinase there is no interference from inhibitors acting on luciferase. Furthermore the standard curve is linerar rather than sigmodial. the Kinase RR Kit is flexible and can easily be adapted to various HTS strategies. Z’ values up to 0.96 have been reported. The assay does not require radioactive materials, antibodies or any form of conjugates.

產(chǎn)品形式

保存建議

4 °C

其他

BioThema成立于1987 年，總部位于瑞典斯德哥爾摩南郊，只做一件事：把螢火蟲熒光素酶反應(yīng)做到極致。38 年來，公司依舊保持“小而專”的規(guī)模，卻憑這一件事成為全球 ATP 與生物冷光分析的隱形冠軍。nBioThema致力于螢火蟲熒光素酶反應(yīng)的分析應(yīng)用。這種多功能反應(yīng)已廣泛用于各種檢測，包括細(xì)胞數(shù)量定量、細(xì)胞增殖、細(xì)胞毒性、參與ATP形成或降解反應(yīng)的所有酶和代謝物分析、報(bào)告基因檢測、免疫檢測、快速細(xì)胞計(jì)數(shù)和環(huán)境衛(wèi)生監(jiān)測。同時(shí)BioThema也是全球很多品牌的ATP和熒光素的OEM原料供應(yīng)商；

注意

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