- 中文名稱
TrueBlack?脂褐素自體熒光淬滅劑，20 X DMF溶液
- 英文名字
- TrueBlack? Lipofuscin Autofluorescence Quencher, 20X in DMF
- 供應(yīng)商
- Biotium
- 產(chǎn)品貨號(hào)
- BTM-23007
- 產(chǎn)品報(bào)價(jià)
- ￥詢價(jià)/1ML
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 90%產(chǎn)品中國有現(xiàn)貨庫存。銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- Features:r
r - Eliminates lipofuscin autofluorescence
r - Reduces autofluorescence from non-lipofuscin sources
r - Doesn't cause high background, unlike Sudan Black B
r - Can be used before or after immunofluorescence staining
r - Clears the way for fluorescence imaging of human and aged animal tissues
r
r TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF, is a new reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy, and accumulate in a variety of cell and tissue types with age. Consequently, imaging of specific immunofluorescence signal in certain adult human tissues or aged animal tissues can be virtually impossible unless methods are employed to quench or mask lipofuscin fluorescence.r r Traditionally, Sudan Black B has been used to quench lipofuscin autofluorescence by incubating tissue sections with the dye after immunofluorescence staining. However, while it masks the autofluorescence from lipofuscin, Sudan Black B also introduces uniform non-specific background fluorescence in the red and far-red channels, limiting the use of fluorescent dyes in those wavelengths. Now Biotium has developed TrueBlack® as a superior alternative to Sudan Black B. TrueBlack® quenches lipofuscin fluorescence with far less increase in red/far-red background fluorescence. TrueBlack® treatment can be performed before or after immunostaining. It is rapid, simple, and has minimal effect on signal from fluorescent antibodies or nuclear counterstains, thus preserving the signal-to-noise ratio of the immunostaining.r r TrueBlack® effectively eliminates lipofuscin autofluorescence in tissues like human and mouse brain and retina.? TrueBlack® also can reduce autofluorescence from other sources, such as collagen, elastin, red blood cells, and general background fluorescence. It is not as effective at quenching these sources of autofluorescence as it is for lipofuscin, but it can improve background in human and non-human tissue types. TrueBlack® also has been used to quench background fluorescence on polycarbonate filters used as cell supports for imaging cytometry.
- 產(chǎn)品描述
- TrueBlack®可有效消除人和小鼠大腦和視網(wǎng)膜等組織中的脂褐素自發(fā)熒光。 TrueBlack®還可以減少來自其他來源的自發(fā)熒光，例如膠原蛋白，彈性蛋白，紅細(xì)胞和一般背景熒光。它在淬滅這些自發(fā)熒光源方面不如它對(duì)脂褐素有效，但它可以改善人類和非人類組織類型中的背景。TrueBlack®還用于猝滅聚碳酸酯濾光片上的背景熒光，所述聚碳酸酯濾光片用作成像細(xì)胞術(shù)的細(xì)胞支持物。
- 產(chǎn)品形式
- 該TrueBlack?脂褐素自體熒光淬滅劑，20 X DMF溶液產(chǎn)品形式待更新請(qǐng)參考產(chǎn)品說明書
- 保存建議
- 室溫
- 其他
- Biotium公司是一家在熒光技術(shù)領(lǐng)域獨(dú)樹一幟的生物技術(shù)公司，公司位于美國加利福尼亞的BAY AREA------中外知名首要的生物科技中心。該公司一直致力于開發(fā)和生產(chǎn)用于生物醫(yī)學(xué)研究的熒光指示劑及其他特殊生化產(chǎn)品。其產(chǎn)品被廣泛應(yīng)用于細(xì)胞生 物學(xué)、蛋白質(zhì)組學(xué)、分子生物學(xué)、免疫學(xué)、微生物學(xué)、診斷學(xué)、生物化學(xué)、神經(jīng)科學(xué)、血液流動(dòng)檢測(cè)及大規(guī)模藥物篩選等眾多領(lǐng)域。Biotium公司專利產(chǎn)品眾多，包括已經(jīng)被廣泛使用的實(shí)時(shí)PCR定量熒光染料EvaGreen、具有劃時(shí)代意義的凝膠核酸染料Gel Red和GelGreen，以及最新推出的Cheetah Taq熱啟動(dòng)酶和CF系列蛋白質(zhì)標(biāo)記染料等。 n目前，Biotium已將其許多專利技術(shù)授權(quán)給一些主要的國際生物技術(shù)公司，包括安捷倫、BD生物科學(xué)、Biolegend、BioRad、EMD Millipore、Illumina、PerkinElmer、Qiagen、Roche、Sartorius AG、Sigma-Aldrich、Thermo Fisher Scientific等。
- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
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