- 中文名稱
PMA，20 mM水溶液
- 英文名字
- PMA, 20mM in H2O
- 供應(yīng)商
- Biotium
- 產(chǎn)品貨號(hào)
- BTM-40019
- 產(chǎn)品報(bào)價(jià)
- ￥詢價(jià)/100UL
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 90%產(chǎn)品中國有現(xiàn)貨庫存。銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- PMA (propidium monoazide) is a high affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other organisms. The dye is weakly fluorescent by itself but becomes more fluorescent after binding to nucleic acids. It preferentially binds to dsDNA with high affinity. Upon photolysis, the dye covalently reacts with DNA, thus resulting in permanent DNA modification. The dye is cell membrane-impermeable, and thus can be selectively used to modify only exposed DNA from dead cells while leaving DNA from viable cells intact. This feature makes the dye highly useful in the selective detection of viable pathogenic cells by quantitative real-time PCR in the presence dead cells whose DNA has been PMA-modified and thus can not be amplified. Since Biotium first developed PMA dye, there have been numerous publications on the use of the dye in pathogenic bacterial detection related to food and water safety, medical diagnosis and biodefense. PMA dye is also available in solid form.
- Molecular weight: 511
- Abs = 464 nm (before photolysis)
- Abs/Em = 510/610 nm (following photolysis and covalent attachment to DNA/RNA)
- Dark red liquid
- Store at 4°C and protect from light at all times
Note: Scientists at Biotium have recently invented a new and improved dye for viability PCR, called PMAxx™. PMAxx™ functions in a manner very similar to PMA, but has much greater activity and ability to distinguish between live and dead bacteria. PMAxx™ can be used as a replacement for PMA in any bacterial viability-PCR assay. We recommend that customers interested in using PMA try PMAxx™ instead, for improved detection of viable bacteria. For viability PCR of gram-negative bacteria we highly recommend using our PMA Enhancer in conjunction with PMA. PMA Enhancer for Gram Negative Bacteria was designed to improve PMA-mediated discrimination between live and dead gram-negative bacteria. When a sequence from a gram-negative bacteria is amplified by PCR, samples pre-treated with Enhancer show a decrease in the signal from dead cells, with no change in the signal from live cells. Biotium also provides strain-specific PMA Real-Time PCR Bacterial Viability kits with validated primers for selected pathogens. For photoactivation of PMA, PMaxx™ or EMA dyes, we recommend the use of Biotium's PMA-Lite™ LED Photolysis Device (for tubes) or Glo-Plate™ Blue LED Illuminator (for plates), which are designed to conduct photolysis under controlled conditions.
- 產(chǎn)品描述
- PMA（單疊氮丙啶）是一種高親和力的光反應(yīng)性DNA結(jié)合染料，由Biotium的科學(xué)家發(fā)明，用于細(xì)菌和其他生物體的活力PCR。染料本身是弱熒光的，但在與核酸結(jié)合后變得更熒光。它優(yōu)先以高親和力結(jié)合dsDNA。在光解時(shí)，染料與DNA共價(jià)反應(yīng)，從而導(dǎo)致永久的DNA修飾。染料是細(xì)胞膜不可滲透的，因此可以選擇性地用于僅修飾來自死細(xì)胞的暴露的DNA，同時(shí)使來自活細(xì)胞的DNA保持完整。該特征使得染料在存在其DNA已被PMA修飾且因此不能擴(kuò)增的死細(xì)胞的情況下通過定量實(shí)時(shí)PCR選擇性檢測活病原細(xì)胞中非常有用。自Biotium首次開發(fā)PMA染料以來，已經(jīng)有許多關(guān)于該染料在與食品和水安全、醫(yī)療診斷和生物防御相關(guān)的病原菌檢測中的使用的出版物。PMA染料也可以固體形式獲得。nbsp;
- 分子量： 511
- Abs = 464 nm（光解前）
- Abs/Em = 510/610 nm（光解和共價(jià)連接到DNA/RNA后）
- 深紅色液體
- 4°C下儲(chǔ)存并始終避光
注：Biotium的科學(xué)家最近發(fā)明了一種新的改進(jìn)的活性PCR染料，稱為PMAxx™。PMAxx™以與PMA非常相似的方式起作用，但具有大得多的活性和區(qū)分活細(xì)菌和死細(xì)菌的能力。PMAxx™可在任何細(xì)菌活力-PCR測定中用作PMA的替代物。我們建議有興趣使用PMA的客戶嘗試PMAxx™，以改善對(duì)活菌的檢測。nbsp; 對(duì)于革蘭氏陰性菌的活力PCR，我們強(qiáng)烈建議將PMA增強(qiáng)劑與PMA結(jié)合使用。設(shè)計(jì)用于革蘭氏陰性菌的PMA增強(qiáng)劑以改善PMA介導(dǎo)的活革蘭氏陰性菌和死革蘭氏陰性菌之間的區(qū)分。當(dāng)通過PCR擴(kuò)增來自革蘭氏陰性細(xì)菌的序列時(shí)，用增強(qiáng)子預(yù)處理的樣品顯示來自死細(xì)胞的信號(hào)減少，而來自活細(xì)胞的信號(hào)沒有變化。nbsp; Biotium還提供菌株特異性PMA實(shí)時(shí)PCR細(xì)菌活力試劑盒，該試劑盒具有針對(duì)選定病原體的經(jīng)過驗(yàn)證的引物。 對(duì)于PMA、PMaxx或EMA染料的光活化™，我們建議使用Biotium的PMA-Lite™LED光解裝置（用于管）或Glo-Plate™藍(lán)色LED照明器（用于板），這些裝置設(shè)計(jì)用于在受控條件下進(jìn)行光解。
- 產(chǎn)品形式
- 該P(yáng)MA，20 mM水溶液產(chǎn)品形式待更新請參考產(chǎn)品說明書
- 保存建議
- 4℃
- 其他
- Biotium公司是一家在熒光技術(shù)領(lǐng)域獨(dú)樹一幟的生物技術(shù)公司，公司位于美國加利福尼亞的BAY AREA------中外知名首要的生物科技中心。該公司一直致力于開發(fā)和生產(chǎn)用于生物醫(yī)學(xué)研究的熒光指示劑及其他特殊生化產(chǎn)品。其產(chǎn)品被廣泛應(yīng)用于細(xì)胞生 物學(xué)、蛋白質(zhì)組學(xué)、分子生物學(xué)、免疫學(xué)、微生物學(xué)、診斷學(xué)、生物化學(xué)、神經(jīng)科學(xué)、血液流動(dòng)檢測及大規(guī)模藥物篩選等眾多領(lǐng)域。Biotium公司專利產(chǎn)品眾多，包括已經(jīng)被廣泛使用的實(shí)時(shí)PCR定量熒光染料EvaGreen、具有劃時(shí)代意義的凝膠核酸染料Gel Red和GelGreen，以及最新推出的Cheetah Taq熱啟動(dòng)酶和CF系列蛋白質(zhì)標(biāo)記染料等。 n目前，Biotium已將其許多專利技術(shù)授權(quán)給一些主要的國際生物技術(shù)公司，包括安捷倫、BD生物科學(xué)、Biolegend、BioRad、EMD Millipore、Illumina、PerkinElmer、Qiagen、Roche、Sartorius AG、Sigma-Aldrich、Thermo Fisher Scientific等。
- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。
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