- 中文名稱
CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) BioAssay ELISA Kit
- 英文名字
- CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) BioAssay ELISA Kit
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- C2446-86
- 產(chǎn)品報(bào)價(jià)
- ￥1/1Kit
- 產(chǎn)品說(shuō)明書
- 點(diǎn)擊查看
- 購(gòu)買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- Vascular cell adhesion molecule-1 (VCAM-1, CD106) is a member of the immunoglobulin gene superfamily. Two forms of VCAM-1 with six or seven extracellular Ig-like domains arise due to alternative splicing from a seven-domain VCAM-1 (7D VCAM-1). 7D VCAM-1 is the dominant form expressed by cultured human endothelial cells (1,2). Homology of domains 1 through 3 with domains 4 through 6 suggests a gene duplication event (1,2). The cDNA of 7D VCAM-1 predicts a core protein of approximately 81kD with seven sites for N-linked glycosylation. Upon complete glycosylation the mature protein has a molecular weight of approximately 102kD. This observation is in general agreement with the 110kD protein immunoprecipitated from cytokine-activated endothelium (3,4).VCAM-1 appears to have been highly conserved through evolution. Both rat and mouse VCAM-1 are highly homologous at the protein level to the human VCAM-1 (77% and 76%, respectively) (5). VCAM-1 supports the adhesion of lymphocytes, monocytes, natural killer cells, eosinophils and basophils, through its interaction with integrin a4b1 (VLA-4). VCAM-1/VLA-4 interaction mediates firm adherence of circulating nonneutrophilic leukocytes to endothelium (6). VCAM-1 also participates in leukocyte adhesion outside of the vasculature, mediating precursor lymphocyte adhesion to bone marrow stromal cells (7) and B cell binding to lymph node follicular dendritic cells (8).VCAM-1 is not constitutively expressed on endothelium, but can be up-regulated in vitro in response to LPS, TNF-a, and IL- 1 (9,10), as well as to interferon-g and IL-4 (11). VCAM-1 is also present on tissue macrophages, dendrite cells, bone marrow fibroblasts, myoblasts and myotubes. A soluble form of VCAM-1 has been described (12,13), and soluble VCAM-1 has been found in serum (14). Increased levels of soluble VCAM-1 can be detected in several disease states, including cancer (15-18), autoimmune disorders (17,19-24), infections (25) and inflammations (17,22,26,27).An anti-VCAM-1 monoclonal antibody is adsorbed onto microwells. Soluble VCAM-1 present in a sample or standard then binds to antibodies adsorbed to the microwells. A mixture of biotin-conjugated monoclonal anti-VCAM-1 antibody and Streptavidin-HRP conjugate is added. Biotinylated anti-VCAM-1 antibody binds to VCAM-1 captured by the first antibody. Streptavidin-HRP binds to the biotinylated anti-VCAM-1. Unbound biotinylated anti-VCAM-1 and Streptavidin-HRP conjugate is removed with a wash step and HRP substrate solution is added to the wells. An amount of colored product is formed, proportional to the amount of soluble VCAM-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from six soluble VCAM-1 standard dilutions and the VCAM-1 sample concentration is determined. The VCAM-1 ELISA is an enzyme-linked immunosorbent assay for quantitative detection of soluble Vascular Cell Adhesion Molecule-1 in cell culture supernatants, serum, plasma, or other biological fluids.Analytical Sensitivity and Detection Limits:Sensitivity: 0.59ng/ml Range of Detection: 3.2-100ng/ml Intra-Assay Variation: 3.1% Inter-Assay Variation: 5.2% Recovery: 89% average Assay Time: 135 minutes Crossreactivity: No interference from other members of the immunoglobulin superfamily. Kit Components:C2446-86A1: 1 aluminum pouch with a Microwell Plate coated with Mab (murine) to human VCAM-1C2446-86A2: 1 vial (0.1ml) Conjugate Mixture containing Biotin-Conjugated Anti-VCAM-1 Mab (murine) mixed with Streptavidin-HRP*C2446-86A3: 2 vials (200ng/ml as reconstituted) soluble VCAM-1 Standard, lyophilized*C2446-86A4: 1 vial lyophilized controlC2446-86A5: 1 bottle (50ml) Wash Buffer Concentrate 20X (PBS, 1% Tween 20)* C2446-86A6: 1 vial (5ml) Assay Buffer Concentrate 20X (PBS, 1% Tween 20, 10% BSA)*C2446-86A7: 1 vial (15ml) Substrate Solution C2446-86A8: 1 vial (12ml) Stop Solution (1M Phosphoric Acid, H3PO4), ready to use C2446-86A9: 1 Microwell Strip HolderC2446-86A10: 2 Adhesive Plate Covers
- 產(chǎn)品特點(diǎn)
- 針對(duì)CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) BioAssay ELISA Kit該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說(shuō)明書。
- 保存建議
- 建議收到CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) BioAssay ELISA Kit產(chǎn)品后將其置于4°C保存。
- 其他
- Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說(shuō)明書為準(zhǔn)。下單前，請(qǐng)瀏覽說(shuō)明書確認(rèn)。
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