Collagen (Rat) Type II, Rat Antibody (IgG) BioAssay ELISA Kit-Collagen (Rat) Type II, Rat Antibody (IgG) BioAssay ELISA Kit代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Collagen (Rat) Type II, Rat Antibody (IgG) BioAssay ELISA Kit

英文名字: Collagen (Rat) Type II, Rat Antibody (IgG) BioAssay ELISA Kit

供應商: USBiological

產(chǎn)品貨號: C7510-34C

產(chǎn)品報價: ￥1/1Kit

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: This ELISA kit is designed to assay type II collagen antibodies in rat sera. The microtiter strips provided in the kit have been coated with rat type II collagen. To reduce non-specific binding, we have incorporated unique blocking agents which inhibit the hydrophobic binding of immunoreactive serum components in sample specimens onto plastic surfaces and thus reduce the background levels.Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600? in length and 15? in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.Heterologous type II collagen is widely used for inducing arthritis in susceptible animals. The serum antibodies in collagen-induced arthritic (CIA) mice are highly specific to the type II collagen used for immunization, however there is significant crossreactivity with other species of type II collagen including rat type II collagen.Kit Components:1. Type II collagen-coated 8-well stripsa) Ten, 8 well strips coated with one species of type II collagen for assaying antibody levels in sample specimens. b) Four, 8 well strips for reference standards. Two strips are used per run allowing two partial plates to be run on two separate occasions.2. Standard antibody (1.2ml, 16units/ml)3. Solution A (Blocking buffer, 15ml)4. Solution B (Sample dilution buffer, 60ml): Use for diluting standards and sample specimens.5. Secondary antibody (two vials of lyophilized, peroxidase-conjugated goat anti-rat IgG): Dissolve one vial in 10ml of solution C (Secondary antibody dilution buffer, 20ml)6. Urea-H2O2 plus buffer tablet containing (two tablets): Dissolve one tablet in 20ml glass-distilled water.7. OPD tablet (two tablets): Dissolve one tablet in 20ml Urea-H2O2 buffer (see step 6 above), just before use.8. Solution D Stop solution: (2.5N Sulfuric acid, 7.5ml)9. Solution E 20X Wash buffer (50ml): dilute in 1 liter of distilled water.

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其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。