- 中文名稱
Copper Assay Kit, BioAssay
- 英文名字
- Copper Assay Kit, BioAssay
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- C7900-59K
- 產(chǎn)品報(bào)價(jià)
- ￥1/250Tests
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- Copper is an essential trace element. Copper-containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin and collagen. Copper is mainly present in caeruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anaemia, hypotonia and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilson's disease, microcytic hypochromic anaemia and bone disease due to reduced collagen synthesis.Simple, direct and automation-ready procedures for measuring copper concentrations find wide applications in research, drug discovery and environmental monitoring. Copper assay kit is designed to measure copper with no or minimal sample treatment. The improved method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured at 359nm, is directly proportional to copper concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.Key Features:Sensitive and accurate. Linear detection range 7 ug/dL (1.0uM) to 300ug/dL (47uM) copper in 96-well plate assay.Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.Applications:Direct Assays: biological, environmental, food and beverage samples.Drug Discovery/Pharmacology: effects of drugs on Cu metabolism.Kit Contents: (250 tests in 96-well plates)Reagent A: 10ml Reagent B: 1.5ml Reagent C: 40mlCopper Standard: 1ml 1.5 mg/dL Cu2+Storage conditions. The kit is shipped at room temperature. Store all reagents at 4 °C. Shelf life: 12 months after receipt. Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Note: metal chelators (e.g. EDTA) interfere with this assay and should be avoided in sample preparation.Procedure using 96-well plate:1. Standards: transfer 100ul dH2O into one Eppendorf tube labeled “Blank”. Into another tube labeled “Standard”, mix 20ul 1.5 mg/dL Standard and 80ul dH2O (final 300 ug/dL Cu2+). Samples: transfer 100ul samples into separate tubes. Add 35ul Reagent A (trichloroacetic acid) to each tube and mix by vortexing. If samples contain protein (e.g. serum/plasma), precipitates form. Centrifuge tubes for 2 min at 14,000 rpm and use clear supernatant for assay. For samples that do not contain protein, the mixture remains clear and centrifugation is not necessary. Transfer 100ul Blank, Standard and Sample into separate wells of a clear flat-bottom 96-well plate.2. For each assay well, prepare Working Reagent by mixing 5ul Reagent B and 150ul Reagent C. Transfer 150ul Working Reagent to each well and tap plate to mix thoroughly.3. Incubate 5 min at room temperature and read optical density at 356-362nm (peak absorbance at 359nm).Note: if sample OD values are higher than the OD value for the 300ug/dL Standard, dilute sample in dH2O and repeat assay. Multiply the results by the dilution factor.Procedure using cuvette:Prepare standards and samples as for 96-Well Assay Procedure::.1. Transfer 400ul Standards and Samples into separate cuvets.2. Add 600ul Working Reagent. Mix by pipetting.3. Incubate 5 min at room temperature and read optical density at 356-362nm (peak absorbance at 359nm).Calculation:The copper concentration of Sample is calculated as=ODSAMPLE–ODBLANKODSTANDARD–ODBLANK× 300 (ug/dL)ODSAMPLE, ODBLANK and ODSTANDARD are optical density values of the Sample, Blank and the 300 ug/dL Standard, respectively.Conversions: 100 ug/dL Cu equals 15.5uM, 0.0001% or 1 ppm.Materials Required, But Not Provided:Pipeting devices and accessories. For 96-well plate assays: clear flat-bottom 96-well plates and plate reader. For cuvet assays: spectrophotometer and cuvets for measuring OD at 356-362nm.General Considerations:For scarce samples (e.g. mice serum or plasma), mix sample with dH2O to a total of 100ul, e.g. 50ul serum+50ul dH2O. Multiply the results by the dilution factor (2 fold).Examples:Human serum, rat plasma, rat serum, and bovine serum were assayed in duplicate using the 96-well plate assay protocol. The copper concentrations were 97 ± 1, 104 ± 1, 101 ± 2, 78 ± 1 ug/dL, respectively.
- 產(chǎn)品特點(diǎn)
- 針對(duì)Copper Assay Kit, BioAssay該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到Copper Assay Kit, BioAssay產(chǎn)品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
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