Cortisol, Saliva, Human, BioAssay ELISA Kit-Cortisol, Saliva, Human, BioAssay ELISA Kit代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Cortisol, Saliva, Human, BioAssay ELISA Kit

英文名字: Cortisol, Saliva, Human, BioAssay ELISA Kit

供應商: USBiological

產(chǎn)品貨號: C7904-22

產(chǎn)品報價: ￥1/1x96Tests

產(chǎn)品說明書: 點擊查看

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背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業(yè)銷售團隊、技術(shù)支持團隊、市場營銷團隊、進出口報關(guān)團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務。我們致力于為您提供有價值的產(chǎn)品和服務，在意您的成功！

產(chǎn)品描述: Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. It is increased under stress situations , physical exercise and external administration of ACTH. Measurement of cortisol levels in general can be used as an indicator of adrenal function and the differential diagnosis of Addison's and Cushing's diseases as well as adrenal hyperplasia and carcinoma. Most circulating cortisol is bound to cortisol binding globulin or transcortin and albumin. The free cortisol, which is considered the active part of blood, is about 1-2%. In the absence of appreciable amounts of the cortisol binding proteins in saliva , salivary cortisol is considered to be free and shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.Intended Use:This kit is intended for the determination of cortisol in human saliva by competitive ELISA. Principle of the Test:The assay is carried out following a typical competitive ELISA format. Competition occurs between an unlabeled antigen (present in standards, control and patient samples) and an enzyme-labeled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of cortisol in the sample. A set of standards is used to plot a standard curve from which the amount of cortisol in patient samples and controls can be directly read.Detection Range:1-100ng/mlSensitivity:1.0ng/mlIntra-Assay: CV <11% Inter-Assay: CV <10%Kit Components:C7904-22A: Microtiter Strip, 1x96 wells. Coated with polyclonal antibody against cortisol. C7904-22B: Cortisol (HRP), 50X, 1x300ul. Cortisol-HRP conjugate in a protein-based buffer with a non-mercury preservative. Preparation: Dilute 1:50 in assay buffer before use (eg. 40ul of HRP in 2 ml of assay buffer). If the whole plate is to be used dilute 240ul of HRP in 12ml of assay buffer. Discard any that is left over.*C7904-22C1: Low Control, 11.1(7.7-12.8ng/ml) 1x600ul*C7904-22C2: High Control, 54.4(38-63.3ng/ml)1x600ul Cortisol in a protein-based buffer with a non-mercury preservative. Prepared by spiking buffer with a defined quantity of cortisol. Refer to vial label for expected value and acceptable range. Stability: Once opened, the control should be used within 14 days or aliquoted and stored frozen. Avoid multiple freezing and thawing cycles. *C7904-22D1: Calibrator A, 0ng/ml cortisol, 1x2ml*C7904-22D2: Calibrator B, 1ng/ml cortisol, 1x600ul*C7904-22D3: Calibrator C, 3ng/ml cortisol, 1x600ul*C7904-22D4: Calibrator D, 10ng/ml cortisol,1x600ul*C7904-22D5: Calibrator E, 30ng/ml cortisol, 1x600ul*C7904-22D6: Calibrator F, 100ng/ml cortisol, 1x600ulNote: Once opened, the calibrators should be used within 14 days or aliquoted and stored frozen. Avoid multiple freezing and thawing cycles.C7904-22E: Wash Buffer, 10X, 1x50mlPreparation: Dilute 1:10 in distilled or deionized water before use. If the whole plate is to be used dilute 50ml of the wash buffer concentrate in 450ml of water.C7904-22F: Assay Buffer, 1x15ml. Ready to use. Protein-based buffer with a non-mercury preservative.C7904-22G: TMB Substrate, 1x16ml. Ready to use. Tetramethylbenzidine and hydrogen peroxide in a non-DMF or DMSO containing buffer.C7904-22H: Stop Solution, 1x6ml. Ready to use. 1M sulfuric acid. Caution: Corrosive! Storage and Stability:Store *C7904-22C and *C7904-22D at 4°C until opened. Once opened aliquot and store at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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保存建議: 建議收到Cortisol, Saliva, Human, BioAssay ELISA Kit產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。