- 中文名稱
C-Peptide ELISA Kit, Human, BioAssay (Hyperproinsulinemia, Insulin Precursor, Proinsulin)
- 英文名字
- C-Peptide ELISA Kit, Human, BioAssay (Hyperproinsulinemia, Insulin Precursor, Proinsulin)
- 供應商
- USBiological
- 產品貨號
- C7905-06
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業(yè)銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應，專業(yè)的產品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- C-Peptide is part of the molecule of Proinsulin, that consists of three parts: C-Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C-Peptide are produced. C-Peptide is released into the blood stream in equal amounts to insulin. A test of C-Peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Although C-Peptide function is not fully understood, it binds to cell membranes which leads to increased intracellular Ca2++, K+ -ATPase and endothelial nitric oxide synthase activities. C-Peptide also functions in repair of the muscular layer of arteries.C-peptide assay measures C-peptide in human serum or other biological fluids.Description:Human insulin and C-Peptide originate as a single polypeptide chain known as proinsulin(MW 9000) in the pancreatic cell. Proinsulin is cleaved proteolytically to form equimolar amounts of mature insulin and C-Peptide that are released into the portal vein. So called because it connects the A and B chains of insulin in the proinsulin molecule. C-Peptide is a single chain of 31 amino acid (MW 30,200). Unlike insulin has no known physiological function. Because C-Peptide has a longer half-life than insulin (2-5 times longer), high concentrations of C-Peptide persist in the peripheral circulation and these level fluctuate less insulin. For these reasons, in plasma C-Peptide concentrations may reflect pancreatic insulin secretion more reliable than the level of insulin itself1. C-Peptide is cleaved from the body by the kidney. Urine concentrations of C-Peptide are 20-50 times higher than in plasma, unlike plasma insulin levels, which fluctuate in response to meals, measurement of the 24 hour urinary excretion of C-Peptide provides a useful monitor of average cell insulin secretion 2. C-Peptide measurements are useful in insulinoma diagnosis, especially in patients treated with insulin. Elevated C-Peptide levels are indicative of insulinoma. C-Peptide measurements are useful in the need for progression to insulin therapy in non-insulin dependent diabetics (NIDDM). C-Peptide measurements are useful as a marker for residual pancreatic tissue after pancreatectomy. It may also be used to monitor the progress of pancreas or islet cell transplantation. C-Peptide measurements are useful in the diagnosis of hypoglycemia brought on by surreptitious insulin administration.Expected Values:It is recommended that each laboratory must determine its own normal and abnormal ranges. Reference Range:A healthy normal population of 100 samples were assayed (0.2-13.2ng/ml).Fasting: 1-3ng/mlSpecificity:High concentrations of lipid or bilirubin do NOT interfere in the insulin assay. Purified hemoglobin up to 50ug/ml has been shown not to interfere the test. No interference for rheumatoid factor or human anti-mouse antibodies (HAMA) was observed. Additional crossreactivity was determined as follows:Human pro-insulin (32- 33 split): 63%Human pro-insulin (64-65 split): 87%Human insulin: 0%Human C-Peptide of Insulin: 100%Fasting concentrations of intact and split pro-insulin are typically only 1-2% of C-Peptide concentrations. Crossreactivity with these molecules is not clinically significant.Species Crossreactivity:This kit has been designed and tested for human serum samples. It may be optimized for other biological fluids. It has not been tested in animals (rat, mouse, etc). Applicability for animal samples will depend upon the crossreactivity of the human antibodies used in the kit with a given animal's hormones/proteins. C-peptide assay measures C-peptide in human serum or other biological fluids.Kit Components:C7905-06A: Microtiter Plate 1x96wellsC7905-06B: Assay Buffer 1x16ml*C7905-06C: Standard 0ng/ml 1x2ml*C7905-06D: Standard 0.3ng/ml1x600ul*C7905-06E: Standard 0.5ng/ml1x600ul*C7905-06F: Standard 2ng/ml1x600ul*C7905-06G: Standard 8ng/ml1x600ul*C7905-06H: Standard 16ng/ml1x600ul*C7905-06J: Control, Low 0.885-1.476ng/ml 1x600ul*C7905-06K: Control, High 5.85-9.75ng/ml 1x600ulC7905-06L: Stop Solution, 1N H2SO4 1x6mlC7905-06M: Wash Solution, 10X 1x50mlC7905-06N: TMB Substrate Complete 1x16mlC7905-06P: C-Peptide (HRP), 100X 1x300ulNOTE: Standards and controls are not stable at 4°C. Aliquot and store at -20°C.Storage and Stability:Store all components except standards and controls at 4°C. Aliquot and store standards C7905-06C - C7905-06H and controls C7905-06J and C7905-06K at -20°C. Stable for a 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對C-Peptide ELISA Kit, Human, BioAssay (Hyperproinsulinemia, Insulin Precursor, Proinsulin)該產品的特點優(yōu)勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到C-Peptide ELISA Kit, Human, BioAssay (Hyperproinsulinemia, Insulin Precursor, Proinsulin)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
-
- 注意
-
該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
-
