Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Human PF4 is used for the proof of heparin-induced thrombocytopenia. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy.
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來(lái)源宿主
大腸桿菌中重組表達(dá)
溶解建議
提供的重組人人血小板因子4(CXCL4), His Tag產(chǎn)品為溶液形式(0.25mg/ml的PF4蛋白溶解于20mM Tris-HCl緩沖液(pH 8.0), 0.2M NaCl, 50%甘油和2mM DTT)形式.