Alprenolol is an antagonist for ?1 and ?2 adrenergic receptors. Alprenolol agarose affinity chromatography was first introduced by Lefkowitz R. J. in 1979 for the purification of erythrocyte ?-adrenergic receptor based on ligand-receptor interactions. It is now routinely used for the purification of functionally active ?1 and ?2-adrenergic receptor. CellMosaic’s SepSphere? Alprenolol Agarose is composed of Agarose 4B Beads linked to alprenolol via a thiol ether linkage.