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細(xì)胞分析試劑盒
中文名稱

Dihydrotestosterone ELISA Kit, BioAssay (5a-DHT, Androstanolone)

英文名字
Dihydrotestosterone ELISA Kit, BioAssay (5a-DHT, Androstanolone)
供應(yīng)商
USBiological
產(chǎn)品貨號
D8036-01
產(chǎn)品報價
¥1/1Kit
產(chǎn)品說明書
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產(chǎn)品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理,是行業(yè)中少有的致力于服務(wù)客戶,幫助客戶,且擁有獨立的專業(yè)銷售團(tuán)隊、技術(shù)支持團(tuán)隊、市場營銷團(tuán)隊、進(jìn)出口報關(guān)團(tuán)隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng),專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù),在意您的成功!
產(chǎn)品描述
The specificity of DHT ELISA kit was determined by measuring interference from high concentrations of the following; DHT (100%), Testosterone (8.7%), 5-beta-DHT (2%), Androstenedione (0.2%), Dehydroepiandrosterone sulfate, 17-beta-estradiol, Estriol, Estrone, Progesterone, 17-OH-Progesterone, Cortical pregnenolone were all <0.01%. DHT kit has been designed and tested for human serum samples. It may be optimized for other biological fluids. It has not been tested in animals (rat, mouse, etc). Since the steroid hormone is the same in all species, this kit should work in most species as long as the sample concentration is within the range of this kit. DHT ELISA kit is based on simultaneous binding of DHT from samples and DHT-HRP conjugate to anti-DHT immobilized on the microtiter well plates. Therefore, free DHT and enzyme-bound DHT compete for limited antibody bound to the pale. In the absence of free DHT, there is a maximum amount of DHT-enzyme bound to the plate. At the end it will produce the maximum color. As the amount of free DHT (samples or std) increases, it reduces the amount of enzyme bound to the plate. The color (blue) produced is inversely proportional to the concentration of DHT in the sample. A std. curve is constructed by generating color from no DHT (std. A, 0pg/ml DHT) to the highest std (Std E, 2500pg/ml DHT). Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of DHT in samples and control is read off the standard curve.Dihydrotestosterone (DHT) (Full name, 5a-Dihydrotestosterone, abbreviating to 5a-DHT, androstanolone) is a biologically active metabolite of the hormone testosterone, formed primarily in the prostate gland, testes, hair follicles, and adrenal glands by the enzyme 5a-reductase by means of reducing the 4,5 double-bond. Dihydrotestosterone belongs to the class of compounds called androgens, also commonly called androgenic hormones or testoids. Androgens are part of the biology of gender by stimulating and controlling the development and maintenance of masculine characteristics. DHT is 3 times more potent than testosterone; testosterone is 5-10 times more potent than adrenal androgens. While DHT is best known for its roles in causing male pattern hair loss and prostate problems, it is crucial to virilization and is necessary to mitigate estrogen's effects in men. DHT is an important contributor to other characteristics generally attributed to males, including facial and body hair growth, and deepening of the voice. Unlike other androgens such as testosterone, DHT cannot be converted by the enzyme aromatase to estradiol. It, therefore, is frequently used in research settings to distinguish between effects of testosterone caused by binding to the androgen receptor, and those caused by testosterone's conversion to estradiol and subsequent binding to estrogen receptors.Samples:Serum. DHT ELISA kit has been used in mouse/rat serum and plasma and culture medium (see published references). Other fluids may be adaptedStandards: 25-2500pg/ml Sample: 50ulSensitivity: 6pg/mlKit Components:D8036-01A: Microtiter Plate 1x96 wellsD8036-01B: Standard 0pg/ml, 1x10mlD8036-01C: Standard 25pg/ml 1x600ulD8036-01D: Standard 100pg/ml 1x600ulD8036-01E: Standard 500pg/ml 1x600ulD8036-01F: Standard 1000pg/ml 1x600ulD8036-01G: Standard 2500pg/ml 1x600ulD8036-01H: DHT Low Control, 30.4-71pg/ml 1x 600ulD8036-01J: Assay Buffer 1x15mlD8036-01K: DHT (HRP), 100X 1x200ulD8036-01L: TMB Substrate 1x16mlD8036-01M: Wash Buffer, 10X 1x50mlD8036-01N: Stop Solution (1N H2SO4)1x6mlD8036-01P: DHT High Control, 317.2-528.6pg/ml 1x600ulStorage and Stability:Store at 4°C. Stable for 6 months after receipt For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產(chǎn)品特點
針對Dihydrotestosterone ELISA Kit, BioAssay (5a-DHT, Androstanolone)該產(chǎn)品的特點優(yōu)勢,歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
保存建議
建議收到Dihydrotestosterone ELISA Kit, BioAssay (5a-DHT, Androstanolone)產(chǎn)品后將其置于4°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶提供了諸多超值選擇。
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