- 中文名稱
Epidermal Growth Factor, Human (EGF), BioAssay ELISA Kit
- 英文名字
- Epidermal Growth Factor, Human (EGF), BioAssay ELISA Kit
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號
- E3374-01
- 產(chǎn)品報價
- ￥1/96Tests
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場營銷團(tuán)隊(duì)、進(jìn)出口報關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- Epidermal growth factor (EGF), a polypeptide mitogen, was first observed in 1959 by Cohen and Levi-Montalcini while studying Nerve Growth Factor (NGF) in snake venom extracts.1 It was subsequently isolated and purified from mouse submandibular glands. When injected into new-born mice this new factor caused precocious eyelid opening and incisor eruption.2,3 EGF was further purified, based on its ability to induce the proliferation of basal skin cells.4 Also, a potent inhibitor of gastric acid secretion was identified and isolated from the urine of a pregnant women, and named human ?-urogastrone. It was shown that this protein was very similar to purified human EGF.5,6This EGF enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal specific for EGF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. EGF if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound EGF and other components of sample. In order to quantitate the amount of EGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for EGF is added to each well to sandwich the EGF immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain EGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.In order to measure the concentration of EGF in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus EGF concentration (pg/ml). The concentration of EGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: Calibrator Diluent I: 20pg/mlCalibrator Diluent II: 15pg/mlRange: 31.3-1000pg/mlKit Components: E3374-01A: Microtiter Plate 1x96wellsE3374-01B:. EGF (HRP) 1x15ml*E3374-01C: EGF Standard 2x4ngE3374-01D: Calibrator Diluent I (serum/plasma) 1x22mlE3374-01E: Calibrator Diluent II (cell culture supernatant/urine) 1x22mlE3374-01F: Wash Buffer (20X) 1x60mlE3374-01G: Substrate A 1x11mlE3374-01H: . Substrate B 1x11mlE3374-01J: Stop Solution, H2SO4, 1x14mlStorage and Stability:Store *E3374-01C powder at 4°C liquid at -20°C. Store at other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產(chǎn)品特點(diǎn)
- 針對Epidermal Growth Factor, Human (EGF), BioAssay ELISA Kit該產(chǎn)品的特點(diǎn)優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到Epidermal Growth Factor, Human (EGF), BioAssay ELISA Kit產(chǎn)品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。
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