- 中文名稱
Estradiol BioAssay ELISA Kit
- 英文名字
- Estradiol BioAssay ELISA Kit
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號
- E3550-02
- 產(chǎn)品報價
- ￥1/1Kit
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊、技術(shù)支持團(tuán)隊、市場營銷團(tuán)隊、進(jìn)出口報關(guān)團(tuán)隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- 17beta-Estradiol is considered the true ovarian hormone in women. It is the most potent estrogen, including Estrone and Estriol. 17beta-Estradiol is responsible for the reproductive epithelia, breast growth, maturation of long bones and development of the secondary female sex characteristics.The 17beta-Estradiol level in plasma is used in assaying for fertility, amenorrhea and precocious puberty in girls.Principle of the Assay:This is an ELISA for the quantitative analysis of Estradiol levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the Estradiol in the sample for a limited number of binding sites on the antibody coated plate.The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate, which generates an optimal color after 30 minutes.Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of Estradiol in the sample or standard. For example, the absence of Estradiol in the sample will result in a bright blue color, whereas the presence of Estradiol will result in decreased or no color development.Usually, urine and tissue culture supernatant can be assayed directly by diluting them with the diluted extraction buffer. Plasma and most other mediums will need to be extracted.Kit Components:E3550-02A: ELISA Buffer: 1x30ml. To be used to dilute enzyme conjugate and Estradiol standards.E3550-02B: Wash Buffer (10X): 1x20ml. To be diluted 1X with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation.E3550-02C: Substrate: 1x20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus hydrogen peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed. Light Sensitive.E3550-02D: Extraction Buffer (5X): 1x30ml. To be diluted 1X with deionized water. This is used for diluting extracted and non-extracted samples.E3550-02E: Estradiol (HRP): 1x150ul. Estradiol HRP concentrate.E3550-02F: Estradiol Standard: 1x100ul. Estradiol standard at the concentration of 1ug/ml.E3550-02G: Estradiol Antibody-Coated Plate: 1x96 well microplate with anti-Estradiol rabbit antibody precoated on each well. The plate is ready for use as is. Do not wash.Storage and Stability:Store kit at 4oC. Stable for at least 6 months.
- 產(chǎn)品特點(diǎn)
- 針對Estradiol BioAssay ELISA Kit該產(chǎn)品的特點(diǎn)優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到Estradiol BioAssay ELISA Kit產(chǎn)品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。
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