Nitric oxide synthases (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. BioVision’s NOS2 ELISA kit is a sandwich ELISA assay for the quantitative measurement of NOS2 in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of NOS2 captured from the samples.
產(chǎn)品特點
Easy, convenient and time-saving method to measure the level of NOS2 in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Detection Range: 0.313 – 20 ng/ml
Sensitivity: < 0.188 ng/ml
Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
No significant cross-reactivity or interference was observed.