Presepsin is a soluble subtype CD14. It is considered a novel biomarker for sepsis diagnostic and prognosis evaluation. BioVision’s Presepsin ELISA kit is a sandwich ELISA assay for the quantitative measurement of Presepsin in human serum, plasma and cell culture supernatants. Anti-Presepsinantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Presepsin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Presepsin amount of sample captured in plate.
產(chǎn)品特點
Easy, convenient and time-saving method to measure the level of Presepsin in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.,No significant cross-reactivity or interference was observed,Sensitivity: < 0.094 ng/ml,Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%,Detection Range: 0.156-10 ng/ml