Phenylethanolamine is a trace amine with a structure similar to those of other trace phenethylamines as well as the catecholamine neurotransmitters. Phenylethanolamine A ELISA Kit is based on Indirect Competitive ELISA method. It can be used to detect PHE A in urine, tissue, feed samples. The micro-plate provided in this kit has been pre-coated with PHE A. During the reaction, PHE A in the samples or standard competes with PHE A on the solid phase supporter for sites of PHE A antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of PHE A. The concentration of PHE A in the samples can be calculated by comparing the OD of the samples to the standard curve.