中文名稱

膠原蛋白雜交肽-生物素偶聯(lián)物（CHP）

英文名字

Collagen Hybridizing Peptide - Biotin conjugate (CHP)

供應(yīng)商

Echelon

產(chǎn)品貨號

EEB-BIO

產(chǎn)品報(bào)價

￥詢價/1mg

產(chǎn)品說明書

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購買方式

銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868。

產(chǎn)品新聞

背景資料

膠原蛋白雜交肽是3-Hisperson Inc.的產(chǎn)品。膠原蛋白雜交肽（CHP）是一種新型獨(dú)特的肽，可在體外和體內(nèi)特異性結(jié)合未折疊的膠原蛋白鏈[1 2 3]。通過共享天然膠原蛋白的Gly-X-Y重復(fù)序列，CHP具有通過以類似于退火至互補(bǔ)DNA鏈的DNA片段的方式重新形成三螺旋結(jié)構(gòu)而與變性膠原蛋白鏈雜交的強(qiáng)能力。CHP非常具體：由于缺乏結(jié)合位點(diǎn)，它對完整的膠原蛋白分子具有可忽略的親和力，并且由于其中性和親水性，它對非特異性結(jié)合是惰性的。CHP是一種強(qiáng)大的組織病理學(xué)工具，能夠直接檢測由多種疾病引起的炎癥和組織損傷以及發(fā)育和衰老期間的組織重塑[3]。CHP可在不使用合成熒光基質(zhì)或遺傳修飾細(xì)胞的情況下，在3D膠原蛋白培養(yǎng)物中通過癌細(xì)胞的蛋白水解遷移而穩(wěn)健地可視化細(xì)胞周基質(zhì)周轉(zhuǎn)[4]。CHP可以在分子水平上測量和定位膠原組織的機(jī)械損傷[5]。它還能夠評估脫細(xì)胞細(xì)胞外基質(zhì)中的膠原蛋白變性[6]。此外，CHP可用于在SDS-PAGE凝膠中特異性顯示膠原蛋白條帶，而無需蛋白質(zhì)印跡[7]。B-CHP用生物素標(biāo)記，用于親和素/鏈霉親和素介導(dǎo)的檢測。B-CHP提供了靈活的檢測選擇，如非綠色熒光和HRP-酶法，以避免背景和增強(qiáng)信號。（目錄號：BIO）用于抗生物素蛋白/鏈霉抗生物素蛋白介導(dǎo)的方法，包括HRP-酶法5-FAM（目錄號：FLU），用于綠色熒光檢測和Cy 3（Catalog #：RED）用于紅色熒光檢測。應(yīng)用：免疫熒光免疫組織化學(xué)細(xì)胞成像SDS-PAGE（凝膠內(nèi)蛋白質(zhì)印跡）運(yùn)輸條件在環(huán)境溫度下以粉末形式快速運(yùn)輸。儲存-20 ° C作為粉末; 4 °C在水中重構(gòu)后無需等分和冷凍。在4 ° C溶液中穩(wěn)定2年。參考文獻(xiàn)1）"Targeting and mimicking collagens via triple helical peptide moleclies" Curr Opin Chem Biol 2013 17 968- 975. 2）“Targeting collagen strand by photo-triggered triple-helix hybridization”Proceedings of the National Academy of Sciences of the United States of America 2012 109 14767-14772.?DOI：10.1073/pnas.1209721109.3）“用膠原雜交肽進(jìn)行組織重塑的原位成像。ACS Nano 2017 11 9825。DOI：10.1021/acsnano.7b031504）“通過肽雜交可視化膠原蛋白水解：從3D細(xì)胞培養(yǎng)到體內(nèi)成像。“Biomaterials 2018 DOI：10.1016/j.biomerials.2018.08.0395）“膠原雜交肽實(shí)現(xiàn)膠原中機(jī)械損傷的分子水平檢測和定位。《國家通訊》 2017 8 14913 DOI：10.1038/ncomms14913.6）“使用膠原雜交肽對脫細(xì)胞組織中的膠原變性進(jìn)行分子評估。“生物材料學(xué)報(bào)。 2017. 53 268-278 DOI：10.1016/j.actbio.2017.01.0797）“Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides” Bioconjugate Chemistry 2013 24 9-16. DOI：10.1021/bc 3005842.酶譜-比酶譜DQ膠原SHG和TEM更信息可靠和方便-對膠原的高親和力和無與倫比的特異性，基本上沒有非特異性結(jié)合-適用于依賴于膠原的二級結(jié)構(gòu)而不是任何定義的結(jié)合序列的來自所有物種的所有類型的膠原-適用于冷凍和石蠟-包埋切片，無需抗原回收-無抗體方法，對任何共染色抗體沒有物種限制小尺寸（2%的IgG分子量），易于組織滲透和整個標(biāo)本染色，無需切片-在4 °C以下的溶液中穩(wěn)定，無需等分儲存

產(chǎn)品描述

 The Collagen Hybridizing Peptides are products of 3-Helix Inc.The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains both in vitro and in vivo [1 2 3]. By sharing the Gly-X-Y repeating sequence of natural collagen CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites and it is inert towards non-specific binding because of its neutral and hydrophilic nature.CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases as well as tissue remodeling during development and aging [3]. CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture without the use of synthetic fluorogenic matrices or genetically modified cells [4]. CHP can measure and localize mechanical injury to collagenous tissue at the molecular level [5]. It also enables assessment of collagen denaturation in decellularized extracellular matrix [6]. In addition CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot [7]. B-CHP is labeled with biotin for avidin/streptavidin-mediated detection. B-CHP offers flexible detection options such as non-green fluorescence and HRP-enzyme methods to avoid background and enhance signal.CHP come in three varieties: labeled with biotin (Catalog #: BIO) for avidin/streptavidin-mediated including HRP-enzyme methods 5-FAM (Catalog #: FLU) for green fluorescence detection and Cy3 (Catalog #: RED) for red fluorescence detection.Applications: immunofluorescence immunohistochemistry cell imaging SDS-PAGE (in-gel Western blot)Shipping ConditionsExpress shipping as powder at ambient temperature. Store at -20 ?°C upon arrival until use.Storage-20 ?°C as powder; 4 ?°C after reconstitution in water no need to aliquot and freeze. Stable for 2 years in solution at 4 ?°C.References1) a€?Targeting and mimicking collagens via triple helical peptide assembliesa€? Curr Opin Chem Biol 2013 17 968-975.2) a€?Targeting collagen strand by photo-triggered triple-helix hybridizationa€? Proceedings of the National Academy of Sciences of the United States of America 2012 109 14767-14772. DOI: 10.1073/pnas.1209721109.3) "In situ imaging of tissue remodeling with collagen hybridizing peptides." ACS Nano 2017 11 9825. DOI: 10.1021/acsnano.7b031504) "Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging." Biomaterials 2018 DOI:10.1016/j.biomaterials.2018.08.0395) "Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides." Nat. Commun. 2017 8 14913 DOI: 10.1038/ncomms14913.6) "Molecular assessment of collagen denaturation in decellularized tissues using the collagen hybridizing peptide." Acta Biomater. 2017. 53 268-278 DOI: 10.1016/j.actbio.2017.01.0797) a€?Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptidesa€? Bioconjugate Chemistry 2013 24 9-16. DOI: 10.1021/bc3005842.Features-More informative reliable and convenient than zymography DQ collagen SHG and TEM-High affinity and unparalleled specificity to collagen with essentially no nonspecific binding-Applicable to all types of collagen from all species relying on collagen's secondary structure instead of any defined sequence for binding-Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval-A non-antibody approach with no species restrictions against any co-staining antibodySmall size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning-Stable in solution under 4 ?°C eliminating the need to aliquot for storage

產(chǎn)品形式

保存建議

-20 °C或以下避光

其他

Echelon 提供1200+精品特色產(chǎn)品，集中圍繞磷脂，磷脂酰肌醇相關(guān)的脂質(zhì)產(chǎn)品，多肽，抗體，活性/抑制劑篩選分析試劑盒，以及篩選結(jié)合的PIP strip，Lipid Strips/Arrays??，為研究治療諸如癌癥、糖尿病、炎癥、感染和心腦血管疾病專家提供全面有效工具。

注意

該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。

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