生物素化Select-HA 50 k是一種透明質(zhì)酸(HA)制劑,采用重組多殺性巴氏桿菌透明質(zhì)酸合酶通過體外合成制備,具有均勻和窄的粒度分布。每個(gè)HA分子用單個(gè)生物素進(jìn)行末端標(biāo)記(還原末端)。生物素化Select-HA 50 k和Select-HATM是Hyalose LLC的商標(biāo)。背景透明質(zhì)酸(HA)是一種高分子量的陰離子多糖(1 000-10 000 kD),由重復(fù)二糖組成,是結(jié)締組織細(xì)胞外基質(zhì)的幾種糖胺聚糖組分之一。游離HA被肝臟吸收,在肝臟中被降解和回收。數(shù)據(jù)表明HA水平之間的關(guān)系,局部炎癥和嚴(yán)重程度的許多疾病,如肝炎B或C類風(fēng)濕性關(guān)節(jié)炎肝纖維化等。儲存-20 °C或以下。建議避免頻繁凍融等分。避免微生物或HA降解酶的污染。DeAngelis P. L. 單分散透明質(zhì)酸聚合物的同步化學(xué)酶促合成。J Biol Chem 2004 279(40)42345-9.
產(chǎn)品描述
Biotinylated Select-HA 50k is a hyaluronic acid (HA) preparation of uniform and narrow size distribution prepared by in vitro synthesis using recombinant Pasteurella multocida hyaluronan synthase. Each HA molecule is end-labeled (reducing terminus) with a single biotin. Biotinylated Select-HA 50k and Select-HAa?¢ is a trademark of Hyalose LLC.BackgroundHyaluronic acid (HA) is a high molecular weight anionic polysaccharide (1 000-10 000 kD) composed of repeating disaccharides and is one of several glycosaminoglycan components of the extracellular matrix of connective tissue. Free HA is taken up by the liver where it is degraded and recycled. Data indicates a relationship between HA levels local inflammation and severity of many disease such as hepatitis B or C rheumatoid arthritis liver fibrosis etc.Storage-20 ?°C or below. Avoid frequent freeze-thaw aliquoting is recommended. Avoid contamination with microbes or HA-degrading enzymes.References1.Jing W.; DeAngelis P. L. Synchronized chemoenzymatic synthesis of monodisperse hyaluronan polymers. J Biol Chem 2004 279 (40) 42345-9.