This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1β. Standards or samples are added to the appropriate micro ELISA plate wells and combined with?the specific antibody. Then a biotinylated detection antibody specific for Human IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain?Human IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns?yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of?Human IL-1β.?The concentration of?Human IL-1β?in samples can be calculated by comparing the OD of the samples to the standard curve.
產(chǎn)品特點
Colorimetric
保存建議
Biomatik品牌廠家建議該人IL-1β(白細(xì)胞介素1Beta)ELISA試劑盒產(chǎn)品,Short term: 4°C; Long term: see manual.