This assay has high sensitivity and excellent specificity for detection of Connexin 43 (CX43). No significant cross-reactivity or interference between Connexin 43 (CX43) and analogues was observed.
產(chǎn)品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuropeptide Y (NPY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuropeptide Y (NPY) and unlabeled Neuropeptide Y (NPY) (Standards or samples) with the pre-coated antibody specific to Neuropeptide Y (NPY). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuropeptide Y (NPY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuropeptide Y (NPY) in the sample.
產(chǎn)品特點(diǎn)
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該大鼠連接蛋白43(CX43)ELISA試劑盒產(chǎn)品,Short term: 4°C; Long term: see manual.