This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 2 Alpha (HIF2a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 2 Alpha (HIF2a) and analogues was observed.
產(chǎn)品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Neurofilament, Light Polypeptide (NEFL) and unlabeled High Sensitive Neurofilament, Light Polypeptide (NEFL) (Standards or samples) with the pre-coated antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the sample.
產(chǎn)品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該大鼠缺氧誘導(dǎo)因子2 Alpha(HIF2a)ELISA試劑盒產(chǎn)品,Short term: 4°C; Long term: see manual.