This assay has high sensitivity and excellent specificity for detection of Insulin Receptor Substrate 2 (IRS2). No significant cross-reactivity or interference between Insulin Receptor Substrate 2 (IRS2) and analogues was observed.
產(chǎn)品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Copper Transporter 1 (COPT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Copper Transporter 1 (COPT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Copper Transporter 1 (COPT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Copper Transporter 1 (COPT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產(chǎn)品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該大鼠胰島素受體底物2(IRS2)ELISA試劑盒產(chǎn)品,Short term: 4°C; Long term: see manual.