This assay has high sensitivity and excellent specificity for detection of Suppressors Of Cytokine Signaling 1 (SOCS1). No significant cross-reactivity or interference between Suppressors Of Cytokine Signaling 1 (SOCS1) and analogues was observed.
產(chǎn)品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitochondrial Trans-2-Enoyl Coenzyme A Reductase (MECR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitochondrial Trans-2-Enoyl Coenzyme A Reductase (MECR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitochondrial Trans-2-Enoyl Coenzyme A Reductase (MECR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitochondrial Trans-2-Enoyl Coenzyme A Reductase (MECR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產(chǎn)品特點(diǎn)
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該大鼠細(xì)胞因子信號(hào)通路抑制劑1(SOCS1)ELISA試劑盒產(chǎn)品,Short term: 4°C; Long term: see manual.