This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 15 (FGF15). No significant cross-reactivity or interference between Fibroblast Growth Factor 15 (FGF15) and analogues was observed.
產(chǎn)品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Matrix Metalloproteinase 2 (MMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Matrix Metalloproteinase 2 (MMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Matrix Metalloproteinase 2 (MMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wide-range Matrix Metalloproteinase 2 (MMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產(chǎn)品特點
Competitive Inhibition
保存建議
Biomatik品牌廠家建議該大鼠成纖維細(xì)胞生長因子15(FGF15)ELISA試劑盒產(chǎn)品,Short term: 4°C; Long term: see manual.