This assay has high sensitivity and excellent specificity for detection of Interleukin 18 (IL18). No significant cross-reactivity or interference between Interleukin 18 (IL18) and analogues was observed.
產(chǎn)品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sex Hormone Binding Globulin (SHBG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sex Hormone Binding Globulin (SHBG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sex Hormone Binding Globulin (SHBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sex Hormone Binding Globulin (SHBG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產(chǎn)品特點(diǎn)
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該大鼠白細(xì)胞介素18(IL18)CLIA試劑盒產(chǎn)品,Short term: 4°C; Long term: see manual.