This assay has high sensitivity and excellent specificity for detection of Nesfatin 1 (NES1). No significant cross-reactivity or interference between Nesfatin 1 (NES1) and analogues was observed.
產(chǎn)品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Amyloid Beta Peptide 1-40 (Ab1-40) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Amyloid Beta Peptide 1-40 (Ab1-40) and unlabeled Amyloid Beta Peptide 1-40 (Ab1-40) (Standards or samples) with the pre-coated antibody specific to Amyloid Beta Peptide 1-40 (Ab1-40). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Amyloid Beta Peptide 1-40 (Ab1-40) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Amyloid Beta Peptide 1-40 (Ab1-40) in the sample.
產(chǎn)品特點(diǎn)
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該大鼠Nesfatin1(NES1)ELISA試劑盒產(chǎn)品,Short term: 4°C; Long term: see manual.