This assay has high sensitivity and excellent specificity for detection of Squamous Cell Carcinoma Antigen 1/2 (SCCA1/SCCA2). No significant cross-reactivity or interference between Squamous Cell Carcinoma Antigen 1/2 (SCCA1/SCCA2) and analogues was observed.
產(chǎn)品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gremlin 1 (GREM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gremlin 1 (GREM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gremlin 1 (GREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gremlin 1 (GREM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產(chǎn)品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該猿鱗狀細胞癌抗原1/2(SCCA1/SCCA2)ELISA試劑盒產(chǎn)品,Short term: 4°C; Long term: see manual.