【PLA2G2E】重組人分泌磷脂酶A2-IIE-Recombinant Human Secreted Phospholipase A2-IIE代理/參數(shù)/廠家-酶-艾美捷科技有限公司

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背景資料: Phospholipase A2（PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid（AA)， a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids， which represent another class of lipid mediators.
The secretory PLA2（sPLA2) family， in which 10 isozymes have been identified， consists of low molecular weight， Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes， such as modification of eicosanoid generation， inflammation， and host defense.This enzyme has been proposed to hydrolyze phosphatidylcholine（PC) in lipoproteins to liberate lyso-PC and free fatty acids in the arterial wall， thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.
In mice， sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in rats.

溶解建議: 提供的人分泌磷脂酶A2-IIE（PLA2G2E）產(chǎn)品為過濾滅菌的凍干粉末；該凍干粉末是從含0.5mg/ml人分泌磷脂酶A2-IIE（PLA2G2E）的0.05M醋酸鹽緩沖液（pH 4）中過濾滅菌并凍干產(chǎn)生的.我們建議加0.2ml 0.1M醋酸鹽緩沖液（pH 4）以配制工作儲存液，使粉末完全溶解.如要使pH值更大，我們建議通過相關(guān)緩沖液進(jìn)一步稀釋到10ug/ml.過高的濃度會影響PLA2G2E的溶解性.

保存建議: 人分泌磷脂酶A2-IIE（PLA2G2E）凍干粉末可置于-20℃凍存用于長期保存.重新溶解后可以在4℃冷藏保存2周.建議分裝以避免反復(fù)凍融.

其他: ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細(xì)胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經(jīng)生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級蛋白，價格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒有添加保護(hù)劑或鹽，這就對工藝提出了更高的要求。同時，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運輸和保存。