中文名稱

【GST】重組谷胱甘肽S-轉(zhuǎn)移酶

英文名字

Recombinant E.Coli Glutathione S-Transferase

供應(yīng)商

ProSpec

產(chǎn)品貨號(hào)

ENZ-393

產(chǎn)品報(bào)價(jià)

￥詢價(jià)/10ug   ￥1mg   ￥50ug

產(chǎn)品說明書

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產(chǎn)品新聞

背景資料

Antioxidant enzyme Glutathione S- Transferase（GST) is thought to do the primary cellular defense mechanism against reactive oxygen species. GST reduces lipid hydroperoxides through its Se-independent glutathione peroxidase activity. The enzyme also detoxifies lipid peroxidation end products such as 4-hydroxynonenal（4-HNE).
The soluble GST is a 26 kDa protein which occurs as a dimer in all aerobic organisms. Each monomer has two domains， one that binds GSH and is an /-structure similar to thioredoxin and the other， all helical， that binds the hydrophobic substrate. The GST -fusion protein expression system is a widely used recombinant protein expression system that allows a peptide or a regulatory protein domain to be expressed as a fusion to the C-terminus of Schistosoma japonicum GST. Fusion proteins also possess GST -enzymatic activity and can undergo dimerization similar to in vivo. The fusion protein can be purified via GST -affinity column chromatography. In most cases， the desired peptides or domains are removed from GST by applying a specific protease that recognizes and cleaves the linker between the protein domain and GST. The technique has been widely used to generate different kinds of proteins for crystallization， molecular immunology studies， the production of vaccines and studies involving protein-protein and protein-DNA interactions.

序列

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR.

來源宿主

大腸桿菌中重組表達(dá)

溶解建議

提供的谷胱甘肽S-轉(zhuǎn)移酶（GST）產(chǎn)品為過濾滅菌的透明溶液；其中谷胱甘肽S-轉(zhuǎn)移酶（GST）產(chǎn)品溶解于磷酸鹽緩沖液 pH 7.4溶液中.

保存建議

谷胱甘肽S-轉(zhuǎn)移酶（GST）溶液可以在-20℃到-80℃溫度下凍存用于長(zhǎng)期保存，保質(zhì)期為12月.建議分裝以避免反復(fù)凍融.

其他

ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗(yàn)，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長(zhǎng)因子、瘦素、干細(xì)胞因子、催乳素、表皮生長(zhǎng)因子、胰島素樣生長(zhǎng)因子、神經(jīng)生長(zhǎng)因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白，價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒有添加保護(hù)劑或鹽，這就對(duì)工藝提出了更高的要求。同時(shí)，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運(yùn)輸和保存。

注意

該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。

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