- 中文名稱(chēng)
FTO, Human, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, KIAA1752)
- 英文名字
- FTO, Human, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, KIAA1752)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- F9103-51
- 產(chǎn)品報(bào)價(jià)
- ￥1/100Tests
- 產(chǎn)品說(shuō)明書(shū)
- 點(diǎn)擊查看
- 購(gòu)買(mǎi)方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類(lèi)最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專(zhuān)業(yè)銷(xiāo)售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷(xiāo)團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專(zhuān)業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- FTO, Fat mass-and obesity-associated gene, was discovered as a responsible gene causing the mouse 'fused toes' mutation. The predicted 502aa Fto protein has a calculated molecular mass of 58kD and contains an N-terminal bipartite nuclear localization signal. FTO is widely expressed in a variety of human tissues, with highest levels in brain and pancreatic islets. Bioinformatics analysis indicates that FTO shares sequence motifs with iron- and 2-oxoglutarate (2OG)-dependent oxygenases. The FTO (human) (IntraCellular) ELISA Kit is to be used for the in vitro quantitative determination of human FTO in cell lysates or cell-based assays (screening). This assay is a sandwich ELISA for quantitative determination of human FTO in cells. A monoclonal antibody specific for FTO has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, FTO is recognized by the addition of a purified polyclonal antibody specific for FTO (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of FTO in the samples. This ELISA is specific for the measurement of natural and recombinant human FTO. It does cross-react with human adiponectin, human RBP4, human Nampt, human vaspin, human progranulin, human resistin, human clusterin, human GPX3, human sirtuin 1, human IDO, human IL-33, human ANGPTL3, human ANGPTL4, human FGF21, mouse progranulin, mouse ANGPTL3, mouse leptin, rat Nampt. The assay range is 0.156-10ng FTO/ml and a detection limit of 50pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).Sample Type:Cell lysates.Intended Use:For the quantitative determination of human FTO in cells.Sensitivity:50pg/ml Range:0.156-10ng FTO/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA for quantitative determination of human FTO in cells. A monoclonal antibody specific for FTO has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, FTO is recognized by the addition of a purified polyclonal antibody specific for FTO (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of FTO in the samples.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlLysis Buffer (10X): 1x12mlDetection Antibody: 1x12mlDetector 100X (HRP conjugated anti-IgG): 1x150ulHuman FTO Standard (lyophilized): 20ngHuman FTO QC Sample (lyophilized): 1 vialSubstrate Solution (TMB): 1x12mlStop Solution: 1x12mlPlate Sealers: 3 eachStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產(chǎn)品特點(diǎn)
- 針對(duì)FTO, Human, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, KIAA1752)該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說(shuō)明書(shū)。
- 保存建議
- 建議收到FTO, Human, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, KIAA1752)產(chǎn)品后將其置于4°C保存。
- 其他
- Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類(lèi)最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說(shuō)明書(shū)為準(zhǔn)。下單前，請(qǐng)瀏覽說(shuō)明書(shū)確認(rèn)。
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