Glutathione (GSH) Assay Kit, BioAssay-Glutathione (GSH) Assay Kit, BioAssay代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Glutathione (GSH) Assay Kit, BioAssay

英文名字: Glutathione (GSH) Assay Kit, BioAssay

供應(yīng)商: USBiological

產(chǎn)品貨號: G8123-47

產(chǎn)品報價: ￥1/250Tests

產(chǎn)品說明書: 點擊查看

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背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨立的專業(yè)銷售團隊、技術(shù)支持團隊、市場營銷團隊、進出口報關(guān)團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù)，在意您的成功！

產(chǎn)品描述: Glutathione is a tripeptide of glycine, glutamic acid and cysteine. In the red blood cell, the reduced form of glutathione is vital in maintaining hemoglobin in a reduced state and hence protecting the cells from oxidative damage. Glutathione is involved in detoxification of hydrogen peroxide through glutathione oxidase. Low levels of glutathione are found in deficiencies of key enzymes involved in glutathione metabolism, such as glucose-6-phosphate dehydrogenase, glutathione synthase and glutathione reductase.Simple, direct and automation-ready procedures for measuring reduced glutathione are becoming popular in Research and Drug Discovery. Glutathione Assay Kit is designed to accurately measure reduced glutathione in biological samples. The improved 5,5'-dithiobis(2-nitrobenzoic acid (DTNB) method combines deproteination and detection (Reagent A) into one reagent. DTNB reacts with reduced glutathione to form a yellow product. The optical density, measured at 412nm, is directly proportional to glutathione concentration in the sample. The optimized formulation has a long shelf life and is completely free of interference due to sample turbidity.Key Features:Sensitive and accurate. Linear detection range 0.4-100uM in 96-well plate.Simple and convenient. The procedure involves mixing the DTNB Reagent with sample, removing protein precipitates for proteinaceous samples, adding a second Reagent and reading the optical density.Low interference. Amino acids and common buffers do not interfere.Applications:Direct Assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts.Drug Discovery/Pharmacology: effects of drugs on glutathione metabolism.Kit Components: (250 tests in 96-well plates)G8123-47A: Reagent A, 1x30ml G8123-47B: Reagent B, 1x30ml G8123-47C: Calibrator, 100uM, 1x10ml (equivalent to 100uM glutathione)Storage and Stability:Store components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Protocol:Important: equilibrate Reagents to room temperature. Shake Reagent A before use.Samples: whole blood samples should be diluted 20-fold with water prior to the assay (n=20). Cell lysate can be prepared as follows: collect 2 x 106 cells by centrifugation at 1,000g for 10 min at 4°C. Wash cells in cold PBS. Lyse cells by homogenization or sonication in 1-2ml of cold buffer containing 50mM MES or phosphate (pH 6-7) and 1mM EDTA. Centrifuge at 10,000g for 15 min at 4°C. Use supernatant for assay.Note: b-mercaptoethanol, dithiothreitol and cysteine are known to interfere in this assay. Avoid using these compounds during sample preparation. Amino acids do not interfere.Procedure using 96-well plate:1. Blank and Calibrator. Transfer 100ul water and 100ul Calibrator into wells of a clear-bottom 96-well plate. Pipette 200ul water into the Blank and Calibrator wells.2. Samples. Whole blood samples should be diluted 20-fold with water prior to the assay (n=20). Deproteination is required for blood, serum, plasma and other proteinaceous samples. Reagent A contains components for both color reaction and deproteination.Mix 120ul sample with 120ul Reagent A in 1.5-mL centrifuge tubes. Vortex to mix well. If turbidity occurs, pellet 5 min at 14,000 rpm in a table centrifuge. If the mixture remains clear, no centrifugation is necessary.3. Transfer 200ul sample/Reagent A mixture into wells of the 96- well plate. Add 100ul Reagent B. Tap plate lightly to mix.4. Incubate 25 min at room temperature. Read OD412nm.Procedure using Cuvet:Mix 400ul sample with 400ul Reagent A, centrifuge sample tubes if precipitation occurs. Transfer 600ul supernatant and mix with 400ul Reagent B. Incubate 25 min at room temperature. Measure OD412nm against water. Transfer 400ul Calibrator and 800ul Water into a clean cuvet and measure OD412nm against water.Calculation:Subtract blank OD (water) from the Calibrator and Sample OD values. The glutathione concentration of Sample is calculated as=ODSAMPLE–ODBLANKODCALIBRATOR–ODBLANKX 100 x n (uM)ODSAMPLE, ODSTD and ODBLANK are optical density values of the sample, Calibrator and sample “Blank” (water or buffer in which the sample was dissolved). n is the dilution factor (20 for blood samples).Conversions: 1 mg/dL glutathione equals 32.5uM, 0.001% or 10 ppm.Materials Required, But Not Provided:Pipeting devices, centrifuge tube and table centrifuge.Procedure using 96-well plate:Clear bottom 96-well plates (e.g. Corning Costar) and plate reader.Procedure using cuvette:Spectrophotometer and cuvets for measuring OD at 412 nm.Example:20ul fresh mouse blood was mixed quickly with 380ul water. Assays in 96-well plate gave blood glutathione concentration of 1124 ± 8uM (n=2)

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其他: Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。