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細(xì)胞分析試劑盒
中文名稱

Glutathione GSH/GSSG Detection BioAssay Kit (Cuvette)

英文名字
Glutathione GSH/GSSG Detection BioAssay Kit (Cuvette)
供應(yīng)商
USBiological
產(chǎn)品貨號
G8124-11
產(chǎn)品報價
¥1/1Kit
產(chǎn)品說明書
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產(chǎn)品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理,是行業(yè)中少有的致力于服務(wù)客戶,幫助客戶,且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場營銷團(tuán)隊(duì)、進(jìn)出口報關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng),專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù),在意您的成功!
產(chǎn)品描述
United States Biological has improved the standard GSH/GSSG Assay Kit by using a new and improved GSH scavenger.Reduced glutathione (GSH) is a tripeptide (gamma-glutamylcysteinylglycine) that contains a free thiol group. GSH is a major tissue antioxidant that provides reducing equivalents for the glutathione peroxidase (GPx) catalyzed reduction of lipid hydroperoxides to their corresponding alcohols and hydrogen peroxide to water:H2O2 + 2GSH ---------> GSSG + 2H2O (catalyzed by GPx)In the GPx catalyzed reaction, the formation of a disulfide bond between two GSH molecules gives rise to oxidized glutathione (GSSG). The enzyme glutathione reductase (GR) recycles GSSG to GSH with the simultaneous oxidation of beta-nicotinamide adenine dinucleotide phosphate (beta-NADPH):GSSG + NADPH -----------> 2GSH + NADP+ (catalyzed by GR)When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease. Therefore, the determination of the GSH/GSSG ratio and the quantification of GSSG are useful indicators of oxidative stress in cells and tissues.Kit Components:G8124-11A: Assay Buffer, 2X, 1x125ml. General buffer used to dilute samples and reagents*G8124-11B: GSSG Standard (10uM), 1x2ml.G8124-11C: Scavenger, 1x3ml. Thiol scavenger to keep GSSG in oxidized form.G8124-11D: DTNB, 4x1vial. Lyophilized 5,5'-dithiobis-2-nitrobenzoic acid.G8124-11E: MPA, 5%, 1x40ml. Metaphosphoric acid used to deproteinate samples.*G8124-11F: Glutathione reductase, 1x130ul. Solution containing recombinant glutathione reductase.*G8124-11G: NADPH, 4x1vial. Lyophilized beta-nicotinamide adenine dinucleotide phosphate.Storage and Stability:Store *G8124-11B, *G8124-11F and *G8124-11G at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產(chǎn)品特點(diǎn)
針對Glutathione GSH/GSSG Detection BioAssay Kit (Cuvette)該產(chǎn)品的特點(diǎn)優(yōu)勢,歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
保存建議
建議收到Glutathione GSH/GSSG Detection BioAssay Kit (Cuvette)產(chǎn)品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶提供了諸多超值選擇。
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