鮭魚/鱒魚IGF-II抗體通過用對應(yīng)于成熟大西洋鮭魚N-末端氨基酸殘基1 - 9的合成肽表位免疫兔來制備( 鮭)胰島素樣生長因子-II。收獲抗血清,并通過疏水電荷誘導(dǎo)層析部分純化IgG級分。該抗體對來自大西洋鮭魚和虹鱒魚的IGF-II具有高度特異性( 梅氏虹鱒)在酶聯(lián)免疫測定中。與鮭魚胰島素的交叉反應(yīng)性約為5%,<0.01%對鮭魚IGF-I和鮭魚GH的影響。 nSalmon/Trout IGF-II antibody was prepared by immunising rabbits with a synthetic peptide epitope corresponding to amino acid residues 1-9 of the N-terminus of mature Atlantic salmon (Salmo salar) IGF-II. The antiserum was harvested and the IgG fraction partially purified by hydrophobic charge induction chromatography. The antibody is highly specific for IGF-II from Atlantic salmon and Rainbow trout (Oncorhynchus mykiss) in an enzyme-linked immunoassay. It has a cross-reactivity of ~ 5% to salmon insulin and < 0.01% to salmon IGF-I and salmon GH.
GroPep Bioreagents公司總部位于澳大利亞,成立于1990年,致力于向廣大科研院所、生物技術(shù)及制藥公司開發(fā)、生產(chǎn)和銷售細胞培養(yǎng)級的活性生長因子(主要集中在胰島素樣生長因子(IGF)領(lǐng)域)、抗體及相關(guān)蛋白試劑,現(xiàn)有產(chǎn)品超過100多個,同時在世界范圍內(nèi)均設(shè)有分銷商。公司在分子生物領(lǐng)域、發(fā)酵學(xué)及重組蛋白和單抗制備方面具有豐富的經(jīng)驗,在制造工藝方面,擁有大量專利技術(shù),GroPep目前已通過AS/NZS ISO 9001:2016質(zhì)量管理體系認(rèn)證。