該IGFBP-1抗體是通過(guò)用衍生自與白喉類(lèi)毒素連接的人IGFBP-1的中心結(jié)構(gòu)域的12個(gè)氨基酸的肽免疫兔而制備的。 該肽序列位于甲狀腺球蛋白1型結(jié)構(gòu)域的緊鄰上游,是IGFBP-1所特有的。 該抗體識(shí)別人IGFBP-1,但不識(shí)別IGFBP-2、3、4、5或6或相關(guān)蛋白。 盡管該序列對(duì)人IGFBP-1是特異性的,但該抗體確實(shí)識(shí)別大鼠和小鼠IGFBP-1,并且也可在許多其它物種中起作用,盡管這應(yīng)通過(guò)實(shí)驗(yàn)確定。 通過(guò)蛋白A層析純化免疫球蛋白級(jí)分。 該抗體適用于免疫測(cè)定、免疫印跡和免疫組織化學(xué)。 This IGFBP-1 antibody was prepared by immunising rabbits with a 12 amino acid peptide derived from the central domain of human IGFBP-1 linked to diphtheria toxoid. The peptide sequence is located immediately upstream of the thyroglobulin type-1 domain and is unique to IGFBP-1. The antibody recognises human IGFBP-1 but not IGFBP-2, 3, 4, 5?or 6 or related proteins. Although the sequence is specific to human IGFBP-1, the antibody does recognise rat and mouse IGFBP-1 and may also work in a number of other species, although this should be experimentally determined. The immunoglobulin fraction was purified by Protein A chromatography. The antibody is suitable for use in immunoassay, immunoblotting and immunohistochemistry.
GroPep Bioreagents公司總部位于澳大利亞,成立于1990年,致力于向廣大科研院所、生物技術(shù)及制藥公司開(kāi)發(fā)、生產(chǎn)和銷(xiāo)售細(xì)胞培養(yǎng)級(jí)的活性生長(zhǎng)因子(主要集中在胰島素樣生長(zhǎng)因子(IGF)領(lǐng)域)、抗體及相關(guān)蛋白試劑,現(xiàn)有產(chǎn)品超過(guò)100多個(gè),同時(shí)在世界范圍內(nèi)均設(shè)有分銷(xiāo)商。公司在分子生物領(lǐng)域、發(fā)酵學(xué)及重組蛋白和單抗制備方面具有豐富的經(jīng)驗(yàn),在制造工藝方面,擁有大量專(zhuān)利技術(shù),GroPep目前已通過(guò)AS/NZS ISO 9001:2016質(zhì)量管理體系認(rèn)證。