該IGFBP-2抗體通過用衍生自與白喉類毒素連接的人IGFBP-2的C末端結(jié)構(gòu)域的15個(gè)氨基酸的肽免疫兔來制備。 肽序列位于C末端上游,是IGFBP-2所特有的。 所述抗體識(shí)別IGFBP-2但不識(shí)別IGFBP-1、3、4、5、6或相關(guān)蛋白。 盡管該序列對人IGFBP-2是特異性的,但該抗體確實(shí)識(shí)別來自幾個(gè)物種的IGFBP-2,盡管這應(yīng)該通過實(shí)驗(yàn)來確定。 通過蛋白A親和層析純化免疫球蛋白級(jí)分。 該抗體適用于免疫測定、免疫印跡和免疫組織化學(xué)。 This IGFBP-2 antibody was prepared by immunising rabbits with a 15 amino acid peptide derived from the C-terminal domain of human IGFBP-2 linked to diphtheria toxoid. The peptide sequence is located upstream of the C terminus and is unique to IGFBP-2. The antibody recognises IGFBP-2 but not IGFBP-1, 3, 4, 5, 6 or related proteins. Although the sequence is specific to human IGFBP-2, the antibody does recognise IGFBP-2 from several species, although this should be experimentally determined. The immunoglobulin fraction was purified by Protein A affinity chromatography. The antibody is suitable for use in immunoassay, immunoblotting and immunohistochemistry.
GroPep Bioreagents公司總部位于澳大利亞,成立于1990年,致力于向廣大科研院所、生物技術(shù)及制藥公司開發(fā)、生產(chǎn)和銷售細(xì)胞培養(yǎng)級(jí)的活性生長因子(主要集中在胰島素樣生長因子(IGF)領(lǐng)域)、抗體及相關(guān)蛋白試劑,現(xiàn)有產(chǎn)品超過100多個(gè),同時(shí)在世界范圍內(nèi)均設(shè)有分銷商。公司在分子生物領(lǐng)域、發(fā)酵學(xué)及重組蛋白和單抗制備方面具有豐富的經(jīng)驗(yàn),在制造工藝方面,擁有大量專利技術(shù),GroPep目前已通過AS/NZS ISO 9001:2016質(zhì)量管理體系認(rèn)證。