中文名稱

【GPHA2】重組人糖蛋白激素a

英文名字

Recombinant Human Thyrostimulin Alpha

供應商

ProSpec

產品貨號

HOR-258

產品報價

￥詢價/10ug   ￥1mg   ￥2ug

產品說明書

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購買方式

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產品新聞

背景資料

Human thyrostimulin ranks among the glycoprotein hormone family. These hormones consist of two subunits， the common alpha- and specific beta-subunits， which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in human: FSH， LH， TSH， and CG. FSH， LH， and TSH， mainly expressed in the anterior pituitary， are essential for coordinated endocrine regulation in the hypothalamus- pituitary axis and show to activate specific G protein–coupled receptors in the thyroid （TSH receptor） and gonads （LH and FSH receptors）， respectively.
The heterodimeric glycoprotein hormones have only been identified in vertebrates and are highly conserved in organisms from primitive rayfin fish （Chondrostei） to human in both primary sequences and functional characteristics.
Corticotroph-derived glycoprotein hormone （CGH）， also referred to as thyrostimulin， is a noncovalent heterodimer of glycoprotein hormone alpha 2 （GPHA2） and glycoprotein hormone beta 5 （GPHB5）.
Recombinant A2/B5 heterodimeric glycoproteins activates human TSH receptors， but not LH and FSH receptors， and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named as thyrostimulin based on its thyroid-stimulating activity. The expression of thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism.

序列

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來源宿主

大腸桿菌中重組表達

溶解建議

提供的重組人糖蛋白激素a產品為白色無菌凍干粉末，推薦使用0.1M 乙酸（pH 4）配制工作液濃度到0.5 mg/ml確保凍干粉完全溶解.由于濃度越高，該蛋白溶解能力越低；如果需要用于更高pH的體系，建議使用相關溶液進一步稀釋到10ug/ml濃度.推薦使用去離子水來配制工作液，濃度大約為0.5 mg/ml，確保凍干粉完全溶解. 產品不是無菌的！在細胞培養(yǎng)前請注意過濾滅菌.

保存建議

Lyophilized Thyrostimulin although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution GPHA2 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.

其他

ProSpec 是一家全球著名細胞因子蛋白生產和研發(fā)的公司，具有20多年的生產經驗，專注于蛋白（重組及合成）生產研發(fā)。我們的核心產品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領先的生產工藝和規(guī)模使其可以提供毫克到克級蛋白，價格優(yōu)于同類公司。絕大部分產品是天然成熟型蛋白，而不含有標簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產品沒有添加保護劑或鹽，這就對工藝提出了更高的要求。同時，ProSpec-Tany絕大部分產品為凍干粉，因此易于運輸和保存。

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

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