中文名稱

【AGRP】重組人刺鼠相關蛋白

英文名字

Recombinant Human Agouti–Related Protein

供應商

ProSpec

產品貨號

HOR-283

產品報價

￥詢價/10ug   ￥1mg   ￥2ug

產品說明書

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購買方式

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產品新聞

背景資料

Agouti-related protein is an endogenous antagonist of hypothalamic alpha-melanocortin receptors MC3R and MC4R with potent orexigenic activity. Although a complete deletion of the AGRP gene does not produce any significant metabolic phenotypes， reduction in AGRP expression by RNA interference is associated with increased metabolic rate along with reduced weight gain. In hypothalamus， it is produced by neurons in the medial portion of arcuate nucleus， which produce also the potent orexigenic peptide Neuropeptide Y （NP-Y）. Another site of central AGRP production is the hypothalamic nucleus. AGRP encompasses 132 amino acid residues and its alpha-melanocortin inhibiting activity results in a 34 amino acid cystine knot domain within the C-terminal （87-132） portion of the protein. Both AGRP and NP-Y expression was shown to be suppressed by leptin. Central administration of AGRP induces hyperphagia and increased gain in body weight in rodents， but may also exert metabolic effects even when hyperphagia is prevented. In the absence of hyperphagia， intracerebralventricular administration of AGRP caused significant increases in plasma leptin and insulin concentrations （twofold and 1.5-fold， respectively） and fat pad mass.
In the periphery， AGRP mRNA was found in adrenal glands， lung， testis， ovary， skeletal muscle and adipose tissue in humans or rodents. In the adrenals， it was shown that AGRP antagonizes glucosteroid production mediated by MC4R. AGRP could then modulate locally the functions of some peripheral tissues such as adrenals.
In human and rat serum， detectable levels of AGRP-like activity were reported in the lower picogram range. The serum AGRP levels were elevated in obese humans compared to lean controls and increased with fasting in rats.

序列

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來源宿主

大腸桿菌中重組表達

溶解建議

提供的重組人刺鼠相關蛋白產品為白色無菌凍干粉末，凍干之前是在5mM TRIS， 25mM NaCl， pH 7.5緩沖液中透析過. 推薦使用去離子水來配制工作液，濃度大約為0.5 mg/ml，確保凍干粉完全溶解.

保存建議

Store lyophilized AGRP protein at -20℃. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4℃ for a limited period of time； it does not show any change after two weeks at 4℃. The lyophilized protein remains stable until the expiry date when stored at -20℃.

其他

ProSpec 是一家全球著名細胞因子蛋白生產和研發(fā)的公司，具有20多年的生產經驗，專注于蛋白（重組及合成）生產研發(fā)。我們的核心產品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領先的生產工藝和規(guī)模使其可以提供毫克到克級蛋白，價格優(yōu)于同類公司。絕大部分產品是天然成熟型蛋白，而不含有標簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產品沒有添加保護劑或鹽，這就對工藝提出了更高的要求。同時，ProSpec-Tany絕大部分產品為凍干粉，因此易于運輸和保存。

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

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