當(dāng)特異性地制備用于體內(nèi)功能測(cè)定時(shí),抗體在體外和體內(nèi)阻斷I型IFN受體信號(hào)傳導(dǎo),而不消耗攜帶IFNAR 1的細(xì)胞。該抗體通過用編碼鼠IFNAR1的胞外結(jié)構(gòu)域的質(zhì)粒對(duì)IFNAR1敲除小鼠進(jìn)行體內(nèi)遺傳免疫來產(chǎn)生。IFNAR1和IFNAR2在幾乎所有細(xì)胞上共表達(dá),并構(gòu)成結(jié)合所有I型IFN(IFN α和IFN β)的異二聚體受體。I型IFN是已經(jīng)顯示在體內(nèi)促進(jìn)抗病毒、抗微生物、抗腫瘤和自身免疫應(yīng)答的細(xì)胞因子家族。 抗小鼠IFNAR 1(MAR 1 - 5A 3)體內(nèi)抗體-超低內(nèi)毒素該產(chǎn)品同種型對(duì)照:Mouse IgG1 Isotype Control for In Vivo - Ultra Low Endotoxin [HKSP] (ICH2247UL) 緩沖液:ICH3001-100ml,nICH3002-100ml,nICH3003-100ml, 濃度:≥ 2.0 mg/ml 純度:>98% by SDS-PAGE and HPLC 內(nèi)毒素:≤ 0.5 EU/mg as determined by the LAL method 制劑:0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
應(yīng)用類型
Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, ELISA
免疫原
This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1.