LO-CD 2a強(qiáng)烈抑制混合淋巴細(xì)胞培養(yǎng)(MLC)3并誘導(dǎo)T細(xì)胞凋亡。此外,LO-CD 2a不誘導(dǎo)靜息T細(xì)胞的增殖,并且抑制由OKT 3抗體誘導(dǎo)的T細(xì)胞增殖,誘導(dǎo)非常低水平的細(xì)胞因子釋放,并且還抑制由OKT 3誘導(dǎo)的細(xì)胞因子產(chǎn)生。LO-CD 2a強(qiáng)烈抑制用可溶性O(shè)KT 3刺激的外周血單核細(xì)胞的增殖,并且誘導(dǎo)人T細(xì)胞低反應(yīng)性。將LO-CD 2a添加至原代MLC抑制T細(xì)胞增殖應(yīng)答,并對(duì)繼發(fā)性免疫應(yīng)答具有后續(xù)抑制作用,從而阻斷原代和記憶性同種異體抗原增殖應(yīng)答。二次MLC中的人T細(xì)胞低反應(yīng)性僅限于同種異體抗原,并且在不存在抗體的情況下和在細(xì)胞表面重新表達(dá)CD 2之后都發(fā)生 抗人CD 2(LO-CD 2a)體內(nèi)抗體原液該產(chǎn)品同種型對(duì)照:Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243) 緩沖液:ICH3001-100ml,nICH3002-100ml,nICH3003-100ml, 濃度:≥ 2.0 mg/ml 純度:>95% by SDS-PAGE 內(nèi)毒素:<1.0 EU/mg as determined by the LAL method 制劑:Sterile, preservative-free, solution in PBS. BSA and Azide free.
應(yīng)用類型
ELISA, Functional Assays, Flow Cytometry
免疫原
LO-CD2a was developed as an immunosuppressive agent for use in organ allotransplantation. LO-CD2a was produced by immunizing a LOU/C rat with purified human T lymphocytes and fusing the splenocytes with the non-secreting rat fusion cell line IR983F, resulting in a rat IgG2b mAb of the IgK-1a allotype