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細(xì)胞分析試劑盒
中文名稱

PCR-Legionella spp Plus檢測試劑盒

英文名字
PCR-Legionella spp Plus Detection Kit
供應(yīng)商
Biovision
產(chǎn)品貨號
K1451-96
產(chǎn)品報(bào)價
¥詢價/96Rxns
產(chǎn)品說明書
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產(chǎn)品描述
PCR-Legionella spp Plus Detection Kit (Legionella spp & Legionella pneumophila) is an ideal tool for an fast and reliable Amplification and Detection of specific DNA fragments from Legionella spp and/or Legionella pneumophila by the real-time PCR method (Multiplex PCR). The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The probe for the detection of target gene is labelled with the FAM (Legionella spp) and HEX fluorochrome (L. pneumophila), whereas the probe for the detection of IAC is labelled with the CY5 fluorochrome. In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
產(chǎn)品特點(diǎn)
Fast: results in less than 24 hours with minimal handling., Inclusivity: Primers and probes have been tested satisfactorily in 14 strains of L. pneumophila and 30 species of the genus Legionella., Amplification limit: Legionella spp: 5 UG per reaction. L. pneumophila: 100 UG per reaction., Detection: probe labelled with fluorescent dyes– Legionella: FAM-BHQ1a; L. pneumophila: HEXBHQ1; IAC:CY5-BHQ2., Quantification limit: Legionella spp: 50 UG/L. L. pneumophila: 1000 UG/L., Exclusivity: No cross-reactions with other frequent bacterial agents have been observed., Simultaneously detect and differentiate Legionella spp. and L. pneumophila in the same tube, Reliable and highly specific Results., Thermal cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers, Quantification Dynamic range: 5 logs, Easy and Ready-to-use Kit.
保存建議
-20
其他
BioVision的公司總部在美麗的舊金山灣地區(qū),它是全球生命科學(xué)公司的領(lǐng)導(dǎo)者.BioVision在開發(fā)和研究細(xì)胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關(guān)特色抗體、重組蛋白及細(xì)胞因子和酶等多種產(chǎn)品,以及其他創(chuàng)新的研究工具.其產(chǎn)品覆蓋細(xì)胞凋亡研究、能量代謝、細(xì)胞/組織蛋白提取、氧化應(yīng)激與損傷、代謝與肥胖癥、細(xì)胞增殖與毒性、信號轉(zhuǎn)導(dǎo)、干細(xì)胞研究等領(lǐng)域.作為多家知名品牌的中國總代理以及中國區(qū)域代理,艾美捷與BioVision一起為研究者們提供最好、最新的細(xì)胞生物學(xué)研究工具而不斷擴(kuò)大產(chǎn)品及服務(wù)質(zhì)量.
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