PCR-STEC檢測(cè)試劑盒-PCR-STEC Detection Kit代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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產(chǎn)品描述: PCR-STEC Detection Kit is an ideal tool for a specific, rapid, and reliable Amplification and Detection of the virulence-associated genes stx1, stx2 and eae by the real-time PCR method (Multiplex PCR). The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The Probe for the detection of target gene is labelled with the FAM (sxt1), HEX (stx2) and ROX (eae) fluorochrome, whereas the probe for the detection of IAC is labelled with the CY5 fluorochrome. In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.

產(chǎn)品特點(diǎn): Thermal cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers., Inclusivity: specifically reacts with all strains of E. coli with the indicated target genes (stx1, stx2 and eae)., Easy and Ready-to-use Kit., Reliable and highly specific Results., Exclusivity: No cross-reactions with other strains of Non- Shiga toxin-producing E. coli (STEC)., Simultaneously detect and differentiate the major STEC-associated virulence marker genes: stx1, stx2 and eae in the same PCR amplification reaction., Quantification Dynamic range: 4 logs, Amplification limit: 5 DNA copies per reaction, Detection: probe labelled with fluorescent dyes– FAM-BHQ1a (sxt1), HEX-BHQ1(stx2), ROX-BHQ2 (eae) and CY5-BHQ1(IAC), Fast: results in less than 24 hours with minimal handling.

其他: BioVision的公司總部在美麗的舊金山灣地區(qū)，它是全球生命科學(xué)公司的領(lǐng)導(dǎo)者.BioVision在開發(fā)和研究細(xì)胞凋亡檢測(cè)分析、代謝檢測(cè)方面具有卓越的影響力.包括檢測(cè)各種檢測(cè)分析試劑盒、相關(guān)特色抗體、重組蛋白及細(xì)胞因子和酶等多種產(chǎn)品，以及其他創(chuàng)新的研究工具.其產(chǎn)品覆蓋細(xì)胞凋亡研究、能量代謝、細(xì)胞/組織蛋白提取、氧化應(yīng)激與損傷、代謝與肥胖癥、細(xì)胞增殖與毒性、信號(hào)轉(zhuǎn)導(dǎo)、干細(xì)胞研究等領(lǐng)域.作為多家知名品牌的中國(guó)總代理以及中國(guó)區(qū)域代理，艾美捷與BioVision一起為研究者們提供最好、最新的細(xì)胞生物學(xué)研究工具而不斷擴(kuò)大產(chǎn)品及服務(wù)質(zhì)量.