明膠酶（明膠降解/ Zymography）分析試劑盒（熒光法）-Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: 明膠酶（明膠降解/ Zymography）分析試劑盒（熒光法）

英文名字: Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric)

供應商: Biovision

產品貨號: K444-100

產品報價: ￥詢價/100assays

產品說明書: 點擊查看

購買方式: 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868.

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背景資料: 待更新

產品描述: Gelatinases are a type of matrix zinc-dependent metalloproteases (MMPs) that degrade gelatins and a variety of other extracellular matrix proteins. These enzymes are synthesized as latent zymogens that are activated by proteolysis and inhibited by tissue inhibitors of metalloproteases (TIMPs). Two mammalian gelatinases, Gelatinase A (MMP-2) and Gelatinase B (MMP-9), are critical for basement membrane degradation and are highly upregulated in variety of tumor cells. Gelatinase activity is usually detected by small peptide-based activity assays which may suffer from lack of substrate specificity. Other methods for gelatinase activity include gelatin Zymography where samples are electrophoresed on a gelatin-containing SDS-PAGE, and further renatured in a suitable buffer for 12-16 h. The zymogram is subsequently stained, and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme. Such methods are laborious, time-consuming and may lead to the loss of enzymatic activity as renaturation may not be completely reversible. BioVision’s Gelatinase Activity Assay Kit utilizes a hybrid approach for the detection of gelatinase activity by employing a highly quenched gelatin substrate which upon cleavage by a suitable gelatinase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of gelatinase activity (as low as 0.06 mCDU for bacterial collagenase) in biological samples.

產品特點: ? Rapid & sensitive ? Simple assay to measure gelatinase activity as well as to screen/study/characterize potential inhibitors of gelatinase ? Includes Fluorometric Gelatinase Substrate and Positive Control

保存建議: -20

其他: BioVision的公司總部在美麗的舊金山灣地區(qū)，它是全球生命科學公司的領導者.BioVision在開發(fā)和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創(chuàng)新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區(qū)域代理，艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務質量.