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細(xì)胞分析試劑盒
中文名稱

PPDK(丙酮酸,磷酸二激酶)活性測(cè)定試劑盒(熒光/比色法)

英文名字
PPDK (Pyruvate, phosphate dikinase) Activity Assay Kit (Fluorometric/Colorimetric)
供應(yīng)商
Biovision
產(chǎn)品貨號(hào)
K456-100
產(chǎn)品報(bào)價(jià)
¥詢價(jià)/100assays
產(chǎn)品說明書
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產(chǎn)品新聞
背景資料
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產(chǎn)品描述
Pyruvate, phosphate dikinase, PPDK, (E.C. 2.7.9.1) is found in microorganisms and plants where it catalyzes reversible interconversion of AMP, PPi and phosphoenolpyruvate (PEP) to ATP, Pi and pyruvate monophosphate. PPDK plays an important role in plant photosynthesis and glycolytic pathway of endobiotic protozoa and bacteria where pyruvate, phosphate dikinase (PK) is absent; it functions in the process of ATP biosynthesis. In plants, PPDK is a pivotal regulator of the C4 photosynthetic cycle and the key limiting factor for maintaining photosynthesis at low temperature. It catalyzes the regeneration of PEP as the primary acceptor of atmospheric CO2 from pyruvate, ATP and phosphate. In addition, PPDK is also present in C3 leaves where it confers a similar light/dark regulation in chloroplasts, and in wheat leaves in an amount comparable to that found in seeds. Since PPDK is not found in vertebrates, finding its inhibitors may be useful for treatment of infections caused by anaerobic protists that depend on pyrophosphate-dependent glycolysis. Specific inhibition of PPDK is expected to inhibit Giardia and other anaerobic protists such as Entamoeba histolytica and Trichomonas vaginalis without harming their mammalian hosts. BioVision’s Pyruvate, phosphate dikinase, (PPDK) Assay Kit provides a simple and rapid test for measuring specific activity of PPDK in bacterial, protozoan and plant lysates as well as protein preps. We utilize the ability of PPDK to interconvert the substrate into intermediate product detected by proprietary enzyme mix and probe. Generated stable product can be quantified by either colorimetric or fluorometric readout. The assay is simple to perform, high-throughput adaptable and a fluorometric reaction can detect as low as of 0.006 U of PPDK activity in a single well. We provide sufficient reagents for 100 fluorometric or colorimetric assays.
產(chǎn)品特點(diǎn)
? Simple procedure; takes ~ less than 60 minutes ? Fast and convenient
保存建議
-20
其他
BioVision的公司總部在美麗的舊金山灣地區(qū),它是全球生命科學(xué)公司的領(lǐng)導(dǎo)者.BioVision在開發(fā)和研究細(xì)胞凋亡檢測(cè)分析、代謝檢測(cè)方面具有卓越的影響力.包括檢測(cè)各種檢測(cè)分析試劑盒、相關(guān)特色抗體、重組蛋白及細(xì)胞因子和酶等多種產(chǎn)品,以及其他創(chuàng)新的研究工具.其產(chǎn)品覆蓋細(xì)胞凋亡研究、能量代謝、細(xì)胞/組織蛋白提取、氧化應(yīng)激與損傷、代謝與肥胖癥、細(xì)胞增殖與毒性、信號(hào)轉(zhuǎn)導(dǎo)、干細(xì)胞研究等領(lǐng)域.作為多家知名品牌的中國總代理以及中國區(qū)域代理,艾美捷與BioVision一起為研究者們提供最好、最新的細(xì)胞生物學(xué)研究工具而不斷擴(kuò)大產(chǎn)品及服務(wù)質(zhì)量.
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