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細(xì)胞分析試劑盒
中文名稱

EZClick?總磷脂測(cè)定試劑盒(基于細(xì)胞)

英文名字
EZClick? Total Phospholipid Assay Kit (Cell-Based)
供應(yīng)商
Biovision
產(chǎn)品貨號(hào)
K537-100
產(chǎn)品報(bào)價(jià)
¥詢價(jià)/100assays
產(chǎn)品說(shuō)明書
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產(chǎn)品描述
Phospholipids are major component of the bilayers of all plasma membranes. A single phospholipid molecule consists of a phosphate group on one (“head”), and two side-by-side chains of fatty acids that make up the "tails”. The phosphate head groups can be modified with organic molecules such as Choline (Cho). Cho-containing phospholipids (Phosphatidylcholines; PC) are critical for structural membrane integrity, cellular metabolism and signaling either as individual molecules or precursors of secondary messengers. Changes in global synthesis of Cho-containing phospholipids are an essential parameter in analysis of cellular response to both, physiological and pathological conditions, environmental stress, or drug treatment. To date, phospholipids biochemistry, cell biology and metabolism remain obscure, due to limited methods for their direct cellular visualization. BioVision’s EZClick? Global Phospholipid Synthesis Assay Kit offers a simple and robust method to label and visualize newly synthesized phospholipids in vivo. Based on the metabolic incorporation of the choline analogs directly into their structure, modified phospholipid molecules can be detected with high sensitivity and spatial resolution by click chemistry with azide-containing dyes (Ex/Em= 494/521 nm). This kit enables quantitative analyses of global biosynthesis/turnover of Cho-containing phospholipids in cells. Cells show strong incorporation of Cho analogs into all classes of phospholipids that can be assayed by microplate reader and fluorescence microscope. The kit provides sufficient materials for 100 assays.
產(chǎn)品特點(diǎn)
Simple, fast, does not require lengthy incubation times
保存建議
-20
其他
BioVision的公司總部在美麗的舊金山灣地區(qū),它是全球生命科學(xué)公司的領(lǐng)導(dǎo)者.BioVision在開(kāi)發(fā)和研究細(xì)胞凋亡檢測(cè)分析、代謝檢測(cè)方面具有卓越的影響力.包括檢測(cè)各種檢測(cè)分析試劑盒、相關(guān)特色抗體、重組蛋白及細(xì)胞因子和酶等多種產(chǎn)品,以及其他創(chuàng)新的研究工具.其產(chǎn)品覆蓋細(xì)胞凋亡研究、能量代謝、細(xì)胞/組織蛋白提取、氧化應(yīng)激與損傷、代謝與肥胖癥、細(xì)胞增殖與毒性、信號(hào)轉(zhuǎn)導(dǎo)、干細(xì)胞研究等領(lǐng)域.作為多家知名品牌的中國(guó)總代理以及中國(guó)區(qū)域代理,艾美捷與BioVision一起為研究者們提供最好、最新的細(xì)胞生物學(xué)研究工具而不斷擴(kuò)大產(chǎn)品及服務(wù)質(zhì)量.
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