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細(xì)胞分析試劑盒
中文名稱

焦磷酸鹽(PPi)分析試劑盒(熒光/比色法)

英文名字
Pyrophosphate (PPi) Assay Kit (Fluorometric/Colorimetric)
供應(yīng)商
Biovision
產(chǎn)品貨號(hào)
K568-100
產(chǎn)品報(bào)價(jià)
¥詢價(jià)/100assays
產(chǎn)品說(shuō)明書(shū)
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產(chǎn)品描述
Inorganic pyrophosphate (diphosphate, pyrophosphoric acid or PPi) is a small molecule formed during, or required as a substrate for, a number of biochemical reactions. Enzymatic reactions that generate PPi include ATP hydrolysis, DNA and RNA polymerization, cyclic AMP formation, and enzymatic activation of fatty acids to form their CoA esters. Conversely, enzymes that utilize PPi may include cyclases, hydrolases and ligases. Measurement of inorganic pyrophosphate (PPi) in plasma, serum and other biologic fluids is important in studies of bone metabolism, renal stone disease, and certain types of arthritis. BioVision’s Pyrophosphate (PPi) Assay Kit provides a fast, convenient and ultrasensitive method for determination of free inorganic pyrophosphate levels in biological material. PPi produced during biotic processes is detected through a series of reactions which utilize a proprietary enzyme mix and probe, generating a stable product that can be quantified by either colorimetric or fluorometric readout. Generated fluorescence (Ex/Em = 535/587 nm) or color (OD: 570 nm) intensities are directly proportional to the concentrations of pyrophosphate, enabling precise measurements. Monomeric inorganic phosphate (Pi) does not interfere with the assay. Our assay delivers an easy and robust method suitable for use in a variety of biological samples and can be performed in a convenient microtiter-plate format. The kit provides sufficient reagents for 100 fluorometric or 50 colorimetric assays, respectively. This kit can detect as low as 1.8 μM PPi in plasma and serum samples.
產(chǎn)品特點(diǎn)
? Simple, sensitive and HTP adaptable Protocol ? Specific for Pyrophosphate: other molecules containing phosphate groups do not interference with the assay ? This kit can detect as little as 2 μM of Pyrophosphate in a variety of biological samples
保存建議
-20
其他
BioVision的公司總部在美麗的舊金山灣地區(qū),它是全球生命科學(xué)公司的領(lǐng)導(dǎo)者.BioVision在開(kāi)發(fā)和研究細(xì)胞凋亡檢測(cè)分析、代謝檢測(cè)方面具有卓越的影響力.包括檢測(cè)各種檢測(cè)分析試劑盒、相關(guān)特色抗體、重組蛋白及細(xì)胞因子和酶等多種產(chǎn)品,以及其他創(chuàng)新的研究工具.其產(chǎn)品覆蓋細(xì)胞凋亡研究、能量代謝、細(xì)胞/組織蛋白提取、氧化應(yīng)激與損傷、代謝與肥胖癥、細(xì)胞增殖與毒性、信號(hào)轉(zhuǎn)導(dǎo)、干細(xì)胞研究等領(lǐng)域.作為多家知名品牌的中國(guó)總代理以及中國(guó)區(qū)域代理,艾美捷與BioVision一起為研究者們提供最好、最新的細(xì)胞生物學(xué)研究工具而不斷擴(kuò)大產(chǎn)品及服務(wù)質(zhì)量.
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