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細胞分析試劑盒
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中文名稱

活性氧(ROS)檢測試劑盒

英文名字
Reactive Oxygen Species (ROS) Detection Assay Kit
供應(yīng)商
Biovision
產(chǎn)品貨號
K936-100
產(chǎn)品報價
¥詢價/100assays
產(chǎn)品說明書
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產(chǎn)品描述
Constant generation of low levels of reactive oxygen species (ROS) and free radicals is a basic feature of all living cells. Low levels of ROS play an essential role in signaling pathways, whereas increased under oxidative stress, ROS activity result in damage to nucleic acids, proteins and membrane lipids. Accumulation of ROS during oxidative stress is also associated with aging, apoptosis or necrosis, and is implicated in pathological conditions such as; vascular diseases, diabetes, renal ischemia, arteriosclerosis, pulmonary disorders, inflammatory diseases, and cancer. Cellular activity of ROS is offset by antioxidants, numerous repair systems, and replacement of damaged DNA. Probes for measuring intracellular ROS levels provide important tools to study oxidative stress inducers and effects of antioxidant therapies. BioVision’s ROS Detection Assay Kit is designed for detection of hydroxyl, peroxyl, or other reactive oxygen species in live cells. We utilize H2DCFDA, a unique cell-permeable fluorogenic probe, compatible with phenol red, FBS and BSA to detect reactive oxygen species in live cells. Upon the cell entry, H2DCFDA is modified by cellular esterases to form a non-fluorescent H2DCF. Oxidation of H2DCF by intracellular ROS yields highly a fluorescent product that can be detected by FACS, microplate reader, or fluorescence microscope (Ex/Em 495/529 nm). The fluorescence intensity is proportional to the ROS levels. Our kit provides a simple and specific assay for the real-time measurement of global levels of ROS in living cells. We include sufficient reagents to perform 100 assays and a common ROS inducer as a control for measurement of ROS levels or antioxidant activity with high sensitivity, specificity and accuracy
產(chǎn)品特點
? Simple & Rapid Protocol ? Convenient: Non-Radioactive, no special handling or disposal required ? High-Throughput ? Accurate: reproducible results with low intra & inter assay variability
保存建議
-20
其他
BioVision的公司總部在美麗的舊金山灣地區(qū),它是全球生命科學(xué)公司的領(lǐng)導(dǎo)者.BioVision在開發(fā)和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關(guān)特色抗體、重組蛋白及細胞因子和酶等多種產(chǎn)品,以及其他創(chuàng)新的研究工具.其產(chǎn)品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應(yīng)激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉(zhuǎn)導(dǎo)、干細胞研究等領(lǐng)域.作為多家知名品牌的中國總代理以及中國區(qū)域代理,艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學(xué)研究工具而不斷擴大產(chǎn)品及服務(wù)質(zhì)量.
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